US 12,391,915 B2
Maintenance of differentiated cells with laminins
Karl Tryggvason, Singapore (SG); Karl Kristian Tryggvason, Stockholm (SE); and Anna Domogatskaya, Ronninge (SE)
Assigned to BioLamina AB, Stockholm (SE)
Filed by BioLamina AB, Sundyberg (SE)
Filed on Oct. 26, 2021, as Appl. No. 17/511,079.
Application 17/511,079 is a continuation of application No. 15/351,124, filed on Nov. 14, 2016, granted, now 11,155,781, issued on Oct. 26, 2021.
Application 15/351,124 is a continuation of application No. 13/866,177, filed on Apr. 19, 2013, granted, now 9,499,794, issued on Nov. 22, 2016.
Claims priority of provisional application 61/754,784, filed on Jan. 21, 2013.
Claims priority of provisional application 61/716,005, filed on Oct. 19, 2012.
Claims priority of provisional application 61/636,293, filed on Apr. 20, 2012.
Claims priority of provisional application 61/636,211, filed on Apr. 20, 2012.
Prior Publication US 2022/0041980 A1, Feb. 10, 2022
This patent is subject to a terminal disclaimer.
Int. Cl. C12N 5/00 (2006.01); C12N 5/071 (2010.01)
CPC C12N 5/0068 (2013.01) [C12N 5/0676 (2013.01); C12N 2501/998 (2013.01); C12N 2533/52 (2013.01)] 25 Claims
OG exemplary drawing
 
1. A method for maintaining the phenotype of a differentiated cell in a cell culture, comprising:
providing a cell culture substrate as a layer on a surface, the layer comprising a laminin, wherein the laminin is an intact protein or a protein fragment;
plating the differentiated cell directly on the cell culture substrate; and
culturing the plated differentiated cell on the layer in a xeno-free and chemically defined cell culture medium, wherein the cell culture medium has a human serum albumin concentration of at least 0.3 mM;
whereby the phenotype of the differentiated cell is maintained in the cell culture;
wherein the laminin is laminin-521 and/or laminin-511, and wherein the differentiated cell is a keratinocyte.