US 12,391,910 B2
Sphere culture member, culture container, method for processing perforated member, and cleaning container
Osamu Koseki, Kanagawa (JP); and Satoshi Tanaka, Kanagawa (JP)
Assigned to TOYO SEIKAN GROUP HOLDINGS, LTD., Tokyo (JP)
Filed by TOYO SEIKAN GROUP HOLDINGS, LTD., Tokyo (JP)
Filed on Apr. 19, 2021, as Appl. No. 17/233,709.
Application 17/233,709 is a continuation of application No. PCT/JP2019/040804, filed on Oct. 17, 2019.
Claims priority of application No. 2018-198005 (JP), filed on Oct. 20, 2018.
Prior Publication US 2021/0238525 A1, Aug. 5, 2021
Int. Cl. C12M 1/12 (2006.01); B29C 43/02 (2006.01); C12M 1/00 (2006.01)
CPC C12M 25/00 (2013.01) [B29C 43/021 (2013.01); C12M 23/26 (2013.01); C12M 23/34 (2013.01); C12M 39/00 (2013.01)] 4 Claims
OG exemplary drawing
 
1. A culture container, which is one culture bag, the one culture bag comprising:
two side walls;
a sphere culture member comprising a film, the sphere culture member being disposed between the two side walls such that a three-layered structure is formed by the two side walls and the sphere culture member,
wherein:
the sphere culture member captures spheres and separates an inside of the container into two spaces between the two side walls;
the sphere culture member has a first surface, a second surface facing away from the first surface, and a plurality of recesses;
in the first surface, a plurality of openings are formed in and corresponding to the plurality of the recesses, respectively;
a plurality of microholes are formed in the second surface, and each of the plurality of microholes communicates with one of the plurality of the openings in the first surface;
a wall surface of the each of the plurality of the recesses is inclined from a corresponding one of the plurality of the openings toward a microhole of the plurality of microholes; and
a circle or inscribed circle of each of the plurality of the openings has a diameter a of 50 μm or more and 1 mm or less, a circle or inscribed circle of each of the plurality of microholes has a diameter b of 1 μm or more and less than 200 μm, and a and b satisfy a>b.