US 12,391,909 B2
Systems and methods for cell culturing
Shashi K. Murthy, Newton, MA (US); and Andrew Kozbial, East Boston, MA (US)
Assigned to FLASKWORKS, LLC, Boston, MA (US)
Filed by FLASKWORKS, LLC, Boston, MA (US)
Filed on Aug. 11, 2022, as Appl. No. 17/886,067.
Application 17/886,067 is a continuation of application No. 17/074,970, filed on Oct. 20, 2020, granted, now 11,434,459.
Claims priority of provisional application 62/923,975, filed on Oct. 21, 2019.
Claims priority of provisional application 62/923,973, filed on Oct. 21, 2019.
Claims priority of provisional application 62/923,967, filed on Oct. 21, 2019.
Claims priority of provisional application 62/923,963, filed on Oct. 21, 2019.
Claims priority of provisional application 62/923,978, filed on Oct. 21, 2019.
Claims priority of provisional application 62/923,982, filed on Oct. 21, 2019.
Prior Publication US 2023/0039516 A1, Feb. 9, 2023
This patent is subject to a terminal disclaimer.
Int. Cl. C12M 1/00 (2006.01); A01N 1/125 (2025.01); A61K 35/17 (2015.01); C12M 1/04 (2006.01); C12M 1/12 (2006.01); C12M 1/26 (2006.01); C12M 1/34 (2006.01); C12M 1/36 (2006.01); C12M 1/42 (2006.01); C12M 3/00 (2006.01); C12N 5/00 (2006.01); C12N 5/0783 (2010.01); C12N 5/0784 (2010.01)
CPC C12M 23/58 (2013.01) [A01N 1/125 (2025.01); A61K 35/17 (2013.01); C12M 23/24 (2013.01); C12M 23/48 (2013.01); C12M 29/00 (2013.01); C12M 29/10 (2013.01); C12M 29/14 (2013.01); C12M 33/12 (2013.01); C12M 35/08 (2013.01); C12M 37/04 (2013.01); C12M 41/14 (2013.01); C12M 41/34 (2013.01); C12M 41/48 (2013.01); C12M 45/22 (2013.01); C12N 5/00 (2013.01); C12N 5/0636 (2013.01); C12N 5/0637 (2013.01); C12N 5/0638 (2013.01); C12N 5/0639 (2013.01); C12N 2501/998 (2013.01); C12N 2502/1121 (2013.01); C12N 2502/1157 (2013.01)] 20 Claims
 
1. A method for expanding T cells, the method comprising:
providing a cell culture instrument comprising a first culture chamber connected to a separate second culture chamber;
generating, in parallel and separate from one another, antigen-presenting moieties in the first culture chamber and activated T cells in the second culture chamber; and
flowing the activated T cells from the second culture chamber into the first culture chamber to culture activated T cells with the antigen-presenting moieties, to thereby produce expanded T cells.