	US 12,391,739 B2
	Method for producing gamma delta T cells
Shin Kaneko, Kyoto (JP); Shoichi Iriguchi, Kyoto (JP); Tatsuki Ueda, Kyoto (JP); Yoshiaki Kassai, Kanagawa (JP); Akira Hayashi, Kanagawa (JP); and Kazuhide Nakayama, Kanagawa (JP)
Assigned to Kyoto University, Kyoto (JP); and Takeda Pharmaceutical Company Limited, Osaka (JP)
Appl. No. 17/259,736
Filed by Kyoto University, Kyoto (JP); and Takeda Pharmaceutical Company Limited, Osaka (JP)
PCT Filed Jul. 12, 2019, PCT No. PCT/JP2019/027697 § 371(c)(1), (2) Date Jan. 12, 2021, PCT Pub. No. WO2020/013315, PCT Pub. Date Jan. 16, 2020.
Claims priority of application No. 2018-133727 (JP), filed on Jul. 13, 2018; and application No. 2019-117891 (JP), filed on Jun. 25, 2019.
Prior Publication US 2021/0130777 A1, May 6, 2021
Int. Cl. C07K 14/725 (2006.01); A61K 39/00 (2006.01); A61K 40/11 (2025.01); A61K 40/31 (2025.01); A61K 40/32 (2025.01); A61K 40/35 (2025.01); A61K 40/42 (2025.01); A61K 48/00 (2006.01); A61P 35/00 (2006.01); C07K 14/54 (2006.01); C07K 16/28 (2006.01); C12N 5/0783 (2010.01); C12N 5/10 (2006.01)

CPC C07K 14/7051 (2013.01) [A61K 40/11 (2025.01); A61K 40/31 (2025.01); A61K 40/4211 (2025.01); A61P 35/00 (2018.01); C07K 14/5443 (2013.01); C12N 5/0636 (2013.01); A61K 2239/48 (2023.05)]

10 Claims

1. A method for producing a γδT cell from an induced pluripotent stem cell, wherein the induced pluripotent stem cell is derived from a cell other than an αβT cell, wherein the method comprises:

(1) establishing an induced pluripotent stem cell from a cell other than an αβT cell,

(2-1) differentiating the induced pluripotent stem cell established in (1) into a hematopoietic progenitor cell,

(2-2) differentiating the hematopoietic progenitor cell obtained from (2-1) into a T cell without using feeder cells, and

(3) introducing into any one of the (i) induced pluripotent stem cell obtained from step (1), (ii) a hematopoietic progenitor cell obtained from step (2-1) and (iii) a T cell obtained from step (2-2):

(a) a nucleic acid encoding a γTCR, and

(b) a nucleic acid encoding a δTCR.