US 12,391,731 B2
Method for modifying gliadin and application thereof
Yixuan Li, Beijing (CN); Pengjie Wang, Beijing (CN); Genna Ba, Beijing (CN); Fazheng Ren, Beijing (CN); Siyuan Liu, Beijing (CN); Ran Wang, Beijing (CN); Longjiao Zhu, Beijing (CN); Rong Liu, Beijing (CN); Bing Fang, Beijing (CN); Ju Qiu, Beijing (CN); Xiaoyu Wang, Beijing (CN); Juan Chen, Beijing (CN); Han Chen, Beijing (CN); Yinhua Zhu, Beijing (CN); Chong Chen, Beijing (CN); Weibo Zhang, Beijing (CN); Xifan Wang, Beijing (CN); and Jingjing He, Beijing (CN)
Assigned to China Agricultural University, Beijing (CN)
Filed by China Agricultural University, Beijing (CN)
Filed on Jan. 15, 2024, as Appl. No. 18/412,625.
Claims priority of application No. 202311132654.5 (CN), filed on Sep. 5, 2023.
Prior Publication US 2025/0074953 A1, Mar. 6, 2025
Int. Cl. C07K 14/415 (2006.01); C07K 1/02 (2006.01); C12N 9/50 (2006.01)
CPC C07K 14/415 (2013.01) [C07K 1/02 (2013.01); C12N 9/50 (2013.01); C12Y 304/21026 (2013.01)] 5 Claims
 
1. A method for modifying a gliadin, comprising the following steps:
completely dissolving gliadin in a solvent with stirring to obtain a first gliadin solution;
adding the first gliadin solution to ultrapure water then stirring to provide a uniformly mixed gliadin solution;
evaporating liquid from the uniformly mixed gliadin solution and adding pure water to a predetermined volume to obtain a second gliadin solution having a mass concentration of gliadin of 5-25 mg/mL;
adding a proline endoprotease to the second gliadin solution at a volume ratio of 1:5-15 and to provide a mass concentration of proline endoprotease of 0.5-2.5 mg/mL to obtain a third gliadin solution for a reaction, and adjusting a pH value of the third gliadin solution during the reaction to neutral according to a gradient comprising:
adjusting the pH value of the third gliadin solution to 5.0±0.2 after adding the proline endoprotease then reacting for 1-1.5 h, then
adjusting the pH value of the third gliadin solution to 6.0±0.2 then continuing to react for 1-1.5 h, then
adjusting the pH value of the third gliadin solution to 7.0±0.2 and then continuing to react for 1-1.5 h; and
thereby obtaining modified gliadin, wherein the modified gliadin has uniform and stable dispersion in water after heating to 85° C. for 10 min or after 15 minutes in a 1.5 M NaCl solution.