	US 12,391,730 B2
	Insecticidal polypeptides having improved activity spectrum and uses thereof
Michi Izumi Willcoxon, Palo Alto, CA (US); and Takashi Yamamoto, Dublin, CA (US)
Assigned to PIONEER HI-BRED INTERNATIONAL, INC., Johnston, IA (US)
Filed by PIONEER HI-BRED INTERNATIONAL, INC., Johnston, IA (US)
Filed on Mar. 30, 2023, as Appl. No. 18/193,151.
Application 18/193,151 is a division of application No. 17/111,115, filed on Dec. 3, 2020, granted, now 11,649,266.
Application 17/111,115 is a continuation of application No. 15/518,677, abandoned, previously published as PCT/US2015/055491, filed on Oct. 14, 2015.
Claims priority of provisional application 62/064,877, filed on Oct. 16, 2014.
Prior Publication US 2023/0331787 A1, Oct. 19, 2023
Int. Cl. C07K 14/325 (2006.01); A01N 37/46 (2006.01); A01N 63/10 (2020.01); A01N 63/50 (2020.01); A01N 65/22 (2009.01); A01N 65/44 (2009.01); C12N 15/82 (2006.01)

CPC C07K 14/325 (2013.01) [A01N 37/46 (2013.01); A01N 63/10 (2020.01); A01N 63/50 (2020.01); A01N 65/22 (2013.01); A01N 65/44 (2013.01); C12N 15/8286 (2013.01); Y02A 40/146 (2018.01)]

12 Claims

1. A recombinant polynucleotide encoding a variant Cry1B polypeptide, wherein the variant Cry1B polypeptide comprises an amino acid sequence having at least 98% sequence identity to SEQ ID NO:29, wherein the polynucleotide is operably linked to a heterologous regulatory element.