US 12,390,810 B2
Electrokinetic microelectrode devices and methods for biomarker analysis
Michael J. Heller, Portland, OR (US); Daniel Heineck, Tigard, OR (US); Stuart D. Ibsen, Portland, OR (US); Sadik Esener, Portland, OR (US); and Jean M. Lewis, San Diego, CA (US)
Appl. No. 17/753,176
Filed by THE REGENTS OF THE UNIVERSITY OF CALIFORNIA, Oakland, CA (US)
PCT Filed Aug. 21, 2020, PCT No. PCT/US2020/047519
§ 371(c)(1), (2) Date Feb. 22, 2022,
PCT Pub. No. WO2021/041259, PCT Pub. Date Mar. 4, 2021.
Claims priority of provisional application 62/891,204, filed on Aug. 23, 2019.
Prior Publication US 2022/0274111 A1, Sep. 1, 2022
Int. Cl. B01L 3/00 (2006.01); A61B 5/157 (2006.01); B03C 5/00 (2006.01); B03C 5/02 (2006.01); C12Q 1/686 (2018.01); G01N 27/447 (2006.01)
CPC B01L 3/502761 (2013.01) [A61B 5/157 (2013.01); B01L 3/502715 (2013.01); B03C 5/005 (2013.01); B03C 5/02 (2013.01); G01N 27/44721 (2013.01); G01N 27/44791 (2013.01); B01L 2200/0647 (2013.01); B01L 2300/0636 (2013.01); B01L 2300/0645 (2013.01); B01L 2400/0415 (2013.01)] 7 Claims
OG exemplary drawing
 
1. A method of analyzing one or more biomarkers in a biofluid, comprising:
obtaining a biofluid from a subject, wherein the biofluid is placed in a collection tube comprising therein an electrokinetic microelectrode array including:
a support structure,
an array of microelectrodes coupled to a first side of the support structure,
a permeation layer at least partially formed on the array of microelectrodes over the support structure, and
one or more detectors, sensors, and/or emitters coupled to a second side of the support structure,
wherein the electrokinetic microelectrode array includes a folded or a sandwiched configuration of the array of microelectrodes on the first side of the support structure and the one or more detectors, sensors, emitters, or combination thereof on the second side of the support structure that forms a microfluidic channel;
contacting the biofluid with the electrokinetic microelectrode array to separate and isolate the one or more biomarkers in the electrokinetic microelectrode array, wherein the electrokinetic microelectrode array separates and isolates the one or more biomarker(s) from the biofluid using one or both of AC dielectrophoretic (DEP) and DC electrophoretic based forces; and
analyzing the one or more biomarkers in the biofluid.