	US 12,390,531 B2
	Colon-targeted active agent delivery carrier and uses thereof
Shih-Kuo Hou, Taipei (TW)
Assigned to AWKEONOMIC BIOTECHNOLOGY CO., LTD., Taipei (TW)
Filed by AWKEONOMIC BIOTECHNOLOGY CO., LTD., Taipei (TW)
Filed on Aug. 1, 2024, as Appl. No. 18/792,077.
Application 18/792,077 is a continuation of application No. 18/367,279, filed on Sep. 12, 2023.
Claims priority of application No. 112134692 (TW), filed on Sep. 12, 2023.
Prior Publication US 2024/0390501 A1, Nov. 28, 2024
Int. Cl. A61K 47/36 (2006.01); A61K 9/00 (2006.01); A61K 9/107 (2006.01); A61K 9/14 (2006.01); A61P 5/50 (2006.01)

CPC A61K 47/36 (2013.01) [A61K 9/0053 (2013.01); A61K 9/107 (2013.01); A61K 9/146 (2013.01); A61P 5/50 (2018.01)]

6 Claims

1. A method for manufacturing hydrogel composite particles, consisting of:

(a) providing an aqueous phase consisting essentially of a Jelly fig low methoxyl pectin, an active agent and an insoluble salt of calcium; wherein the Jelly fig low methoxyl pectin has the characteristics of:

(1) an average molecular weight of at least 750,000 daltons;

(2) an esterification degree of about 31% or less; and

(3) a galacturonic acid content of at least 75% to about 90%;

(b) mixing the aqueous phase with an oil phase to form a water-in-oil emulsion;

(c) dripping an acid into the water-in-oil emulsion, such that the insoluble salt of calcium dissolves to release calcium ion, and the Jelly fig low methoxyl pectin crosslinks with calcium ion to form hydrogel composite particles containing the active agent;

(d) pouring a critical volume of an aqueous solution consisting essentially of a soluble salt of calcium into the water-in-oil emulsion to solidify the hydrogel composite particles and to de-emulsify the water-in-oil emulsion into the aqueous phase and the oil phase, wherein the hydrogel composite particles are in the aqueous phase;

(d1) decanting the oil phase; and

(e) separating the hydrogel composite particles from the aqueous phase by centrifugation or filtration, wherein the hydrogel composite particles consists essentially of calcium pectinate, the active agent and water.