US 12,390,146 B2
Reagents and methods for modulating cone photoreceptor activity
Jay Neitz, Seattle, WA (US); Maureen Neitz, Seattle, WA (US); James A. Kuchenbecker, Seattle, WA (US); and William W. Hauswirth, Gainesville, FL (US)
Assigned to UNIVERSITY OF WASHINGTON, Seattle, WA (US); and UNIVERSITY OF FLORIDA RESEARCH FOUNDATION, INCORPORATED, Gainesville, FL (US)
Filed by UNIVERSITY OF FLORIDA RESEARCH FOUNDATION, INCORPORATED, Gainesville, FL (US); and UNIVERSITY OF WASHINGTON, Seattle, WA (US)
Filed on Oct. 13, 2021, as Appl. No. 17/500,426.
Application 17/500,426 is a continuation of application No. 15/939,674, filed on Mar. 29, 2018, granted, now 11,160,488.
Application 15/939,674 is a continuation of application No. 14/837,448, filed on Aug. 27, 2015, abandoned.
Application 14/837,448 is a continuation of application No. 14/075,415, filed on Nov. 8, 2013, granted, now 9,198,595, issued on Dec. 1, 2015.
Application 14/075,415 is a continuation of application No. 13/395,609, abandoned, previously published as PCT/US2010/048964, filed on Sep. 15, 2010.
Claims priority of provisional application 61/242,587, filed on Sep. 15, 2009.
Prior Publication US 2022/0183613 A1, Jun. 16, 2022
Int. Cl. C12N 15/86 (2006.01); A61B 5/00 (2006.01); A61B 5/398 (2021.01); A61K 48/00 (2006.01); A61N 5/06 (2006.01); C07K 14/005 (2006.01); C12N 7/00 (2006.01); C12N 15/85 (2006.01)
CPC A61B 5/398 (2021.01) [A61B 5/0036 (2018.08); A61B 5/0048 (2013.01); A61B 5/4836 (2013.01); A61B 5/4848 (2013.01); A61B 5/6821 (2013.01); A61B 5/7225 (2013.01); A61K 48/0058 (2013.01); A61K 48/0075 (2013.01); A61N 5/0622 (2013.01); C07K 14/005 (2013.01); C12N 7/00 (2013.01); C12N 15/85 (2013.01); C12N 15/86 (2013.01); A61N 2005/0652 (2013.01); A61N 2005/0661 (2013.01); A61N 2005/0663 (2013.01); C12N 2750/14132 (2013.01); C12N 2750/14143 (2013.01); C12N 2799/025 (2013.01); C12N 2830/008 (2013.01); C12N 2830/42 (2013.01); C12N 2840/44 (2013.01)] 10 Claims
 
1. An isolated nucleic acid expression vector comprising:
(a) a promoter region comprising an M opsin promoter comprising the nucleic acid sequence of SEQ ID NO: 2, wherein the promoter region is specific for primate retinal cone cells; and
(b) a gene encoding a therapeutic protein comprising the amino acid sequence of SEQ ID NO: 11, wherein the gene is operatively linked to the promoter region.