US 12,389,848 B2
Stevia plant having high rebaudioside M content ratio and screening method for same
Tadayoshi Hirai, Kyoto (JP); Kazunari Iwaki, Kanagawa (JP); Kentaro Ochiai, Kyoto (JP); Saori Takeyama, Kanagawa (JP); and Katsuro Miyagawa, Kyoto (JP)
Assigned to SUNTORY HOLDINGS LIMITED, Osaka (JP)
Appl. No. 17/924,532
Filed by SUNTORY HOLDINGS LIMITED, Osaka (JP)
PCT Filed May 11, 2021, PCT No. PCT/JP2021/017954
§ 371(c)(1), (2) Date Nov. 10, 2022,
PCT Pub. No. WO2021/230257, PCT Pub. Date Nov. 18, 2021.
Claims priority of application No. 2020-084129 (JP), filed on May 12, 2020.
Prior Publication US 2023/0225273 A1, Jul. 20, 2023
Int. Cl. A01H 6/14 (2018.01); A23L 27/30 (2016.01); A23L 33/105 (2016.01); C07H 15/256 (2006.01); C12Q 1/6895 (2018.01)
CPC A01H 6/1488 (2018.05) [A23L 27/36 (2016.08); A23L 33/105 (2016.08); C07H 15/256 (2013.01); C12Q 1/6895 (2013.01); C12Q 2600/13 (2013.01)] 17 Claims
 
1. A method of screening for a stevia plant with high rebaudioside M content ratio, comprising detecting from the genome of a test stevia plant the presence and/or the absence of at least one of the following genetic features (1) and (2), and the presence and/or the absence of at least one of the following genetic features (3) to (7):
(1) homozygous for the allele wherein the base at the position corresponding to position 290 of SEQ ID NO: 1 is T;
(2) homozygous for the allele wherein the base at the position corresponding to position 33 of SEQ ID NO: 2 is A;
(3) homozygous for the allele wherein the base at the position corresponding to position 44 of SEQ ID NO: 3 is T;
(4) homozygous for the allele wherein the base at the position corresponding to position 40 of SEQ ID NO: 4 is T;
(5) homozygous for the allele wherein the base at the position corresponding to position 48 of SEQ ID NO: 5 is C;
(6) homozygous for the allele wherein the portion corresponding to positions 55-72 of SEQ ID NO: 6 is deleted;
(7) homozygous for the allele wherein the base at the position corresponding to position 50 of SEQ ID NO: 7 is A.