	US 12,385,906 B2
	Methods of detection of compound, antibody or protein using recombinant endospores or bacteria as sensing element
Shao-Yi Hou, Taipei (TW)
Assigned to NATIONAL TAIPEI UNIVERSITY OF TECHNOLOGY, Taipei (TW)
Filed by NATIONAL TAIPEI UNIVERSITY OF TECHNOLOGY, Taipei (TW)
Filed on Feb. 14, 2022, as Appl. No. 17/670,818.
Application 17/670,818 is a continuation of application No. 16/813,028, filed on Mar. 9, 2020, granted, now 11,275,082.
Prior Publication US 2022/0236260 A1, Jul. 28, 2022
Int. Cl. G01N 33/543 (2006.01); C12Q 1/02 (2006.01)

CPC G01N 33/54306 (2013.01) [C12Q 1/02 (2013.01)]

17 Claims

1. A system for detecting the presence of an analyte in a sample, comprising (a) a recombinant Escherichia coli (E. coli) expressing one or more identical or different recombinant proteins on the surface thereof, wherein at least one of the recombinant proteins specifically binds to the analyte directly or through a binding agent that specifically binds to said recombinant protein and the analyte, and (b) a signal-producing substance that can be detected, and wherein the system is a diagnostic kit.