US 12,385,102 B2
Assays and methods for determining microbial resistance
Maria Torres-Gonzalez, Omaha, NE (US); Nancy Hanson, Omaha, NE (US); Joel Lechner, Omaha, NE (US); Stephanie Cossette, Omaha, NE (US); Cathy Scheer, Omaha, NE (US); Matthew R. Kreifels, Elkhorn, NE (US); Stacey Morrow, Omaha, NE (US); Christopher Connelly, Gretna, NE (US); Laura R. Porter, Omaha, NE (US); and Randy Fowler, Broomfield, CO (US)
Assigned to STRECK, LLC, La Vista, NE (US); and CREIGHTON UNIVERSITY, Omaha, NE (US)
Filed by STRECK LLC, La Vista, NE (US); and CREIGHTON UNIVERSITY, Omaha, NE (US)
Filed on Jun. 9, 2023, as Appl. No. 18/332,590.
Application 18/332,590 is a division of application No. 16/310,074, granted, now 11,708,614, previously published as PCT/US2017/037700, filed on Jun. 15, 2017.
Claims priority of provisional application 62/350,457, filed on Jun. 15, 2016.
Prior Publication US 2024/0150850 A1, May 9, 2024
Int. Cl. C12Q 1/689 (2018.01); C12Q 1/6809 (2018.01); C12Q 1/6837 (2018.01); C12Q 1/6851 (2018.01); C12Q 1/686 (2018.01)
CPC C12Q 1/689 (2013.01) [C12Q 1/6809 (2013.01); C12Q 1/6837 (2013.01); C12Q 1/6851 (2013.01); C12Q 1/686 (2013.01); C12Q 2545/101 (2013.01); C12Q 2600/106 (2013.01); C12Q 2600/16 (2013.01); C12Q 2600/166 (2013.01)] 10 Claims
 
1. A kit for identification of one or more β-lactamase genes, wherein the one or more β-lactamase genes are selected from the group consisting of: CMY, CTX-M, OXA, IMP, VIM, DHA, KPC, and NDM genes, the kit comprising probes comprising the following sequences: ACGCTAACTCCAGCATTGGTCTGT (SEQ ID NO: 154), AACTTGCCGAATTAGAGCRGCAGT (SEQ ID NO: 157), TATCATTGGTGGTGCCGTAGTCGC (SEQ ID NO: 160), TGAGACACGGTCCAGACTCCTACG (SEQ ID NO: 163), TAGCTTGATCGCCCTCGATTTGGG (SEQ ID NO: 166), TTRTTYGGTGGTTGYTTTRTTAA (SEQ ID NO: 169), CCGGACGGTCTTGGTAATTTGGGT (SEQ ID NO: 172), CCGTACGGTTTAGGCAATTTGGGT (SEQ ID NO: 173), AGCTCTTCTATCCTGGTGCTGCG (SEQ ID NO: 176), TGAGACACGGTCCAGACTCCTACG (SEQ ID NO: 179), AAACCGGGCGATATGCGTCTGTAT (SEQ ID NO: 182), TCGTCGCGGAACCATTCGCTAAA (SEQ ID NO: 185), CATCAGGACAAGATGGGCGGTATG (SEQ ID NO: 188), and TGAGACACGGTCCAGACTCCTACG (SEQ ID NO: 191), wherein each probe comprises a fluorophore and/or a fluorescent quencher.