| CPC C12Q 1/686 (2013.01) [C12Q 1/6851 (2013.01); C12Y 207/07007 (2013.01)] | 20 Claims |
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1. A method, comprising:
a) providing a reaction mixture comprising:
i) a sample suspected of comprising a target nucleic acid;
ii) purified exogenous non-target DNA from fish;
iii) DNA polymerase;
iv) dNTPs;
v) amplification primers configured for amplifying a DNA product from the target nucleic acid;
vi) flap endonuclease; and
vii) an oligonucleotide probe hybridizable to the DNA product;
wherein the reaction mixture is characterized in that it can amplify the DNA product from the target nucleic acid if the target nucleic acid is present in the sample; and
b) conducting an amplification reaction with the reaction mixture wherein, if the target nucleic acid is present in the reaction mixture, an oligonucleotide probe hybridized to the DNA product is cleaved by the flap endonuclease.
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