	US 12,385,067 B2
	Compositions and therapeutic methods of microRNA gene delivery
Pierpaolo Peruzzi, Newton, MA (US); and Ennio Antonio Chiocca, Boston, MA (US)
Assigned to The Brigham and Women's Hospital, Inc., Boston, MA (US)
Filed by The Brigham and Women's Hospital, Inc., Boston, MA (US)
Filed on Feb. 14, 2024, as Appl. No. 18/441,280.
Application 18/441,280 is a continuation of application No. 17/051,527, granted, now 11,946,064, previously published as PCT/US2019/029988, filed on Apr. 30, 2019.
Claims priority of provisional application 62/664,362, filed on Apr. 30, 2018.
Prior Publication US 2024/0240202 A1, Jul. 18, 2024
Int. Cl. C12N 15/86 (2006.01); A61K 45/06 (2006.01); A61K 48/00 (2006.01); C12N 15/113 (2010.01); C12Q 1/68 (2018.01)

CPC C12N 15/86 (2013.01) [A61K 45/06 (2013.01); A61K 48/005 (2013.01); C12N 15/113 (2013.01); C12N 2310/11 (2013.01); C12N 2310/141 (2013.01); C12N 2310/16 (2013.01); C12N 2310/531 (2013.01); C12N 2320/30 (2013.01); C12N 2330/50 (2013.01); C12N 2750/14143 (2013.01)]

35 Claims

1. A composition comprising a non-naturally occurring microRNA cluster composition, the cluster comprising:

(i) a 5′ flanking sequence;

(ii) two or more microRNA hairpin domains, wherein the two or more hairpin domains are separated by one or more spacer sequences;

(iii) two or more pairs of acceptor sites attached to the two or more microRNA hairpin domains, wherein the two or more pairs of acceptor sites are heterologous to said microRNA hairpin domains; and

(iv) a 3′ flanking sequence.