	US 12,385,005 B2
	Compositions and methods for improved malonyl-CoA biosynthesis using 2-stage dynamic metabolic control
Michael Lynch, Durham, NC (US); and Jeovanna Rios, Durham, NC (US)
Assigned to Duke University, Durham, NC (US)
Filed by Duke University, Durham, NC (US)
Filed on Mar. 21, 2023, as Appl. No. 18/187,102.
Claims priority of provisional application 63/269,646, filed on Mar. 21, 2022.
Prior Publication US 2024/0132833 A1, Apr. 25, 2024 Prior Publication US 2024/0228945 A9, Jul. 11, 2024
Int. Cl. C12N 1/20 (2006.01); C07K 14/245 (2006.01); C12N 9/88 (2006.01); C12N 15/113 (2010.01); C12N 15/52 (2006.01); C12N 15/70 (2006.01); C12P 7/46 (2006.01); C12R 1/19 (2006.01)

CPC C12N 1/205 (2021.05) [C12N 9/88 (2013.01); C12N 15/113 (2013.01); C12N 15/52 (2013.01); C12R 2001/19 (2021.05); C12Y 401/01009 (2013.01)]

9 Claims

1. A genetically modified microorganism comprising:

a first synthetic metabolic valve for dynamic and selective regulation of a nitrogen regulatory protein (glnB), the synthetic metabolic valve comprising:

gene expression-silencing of a glnB gene,

selective enzymatic degradation of a glnB protein, or

a combination thereof,

wherein the synthetic metabolic valve is selectively activated to silence a glnB gene and/or degrade a glnB protein in response to depletion of phosphate as a limiting nutrient in the growth media in which the genetically modified microorganism is growing.