US 12,383,904 B2
Device for manipulating samples
Leon Chew, Vancouver (CA)
Assigned to STEMCELL TECHNOLOGIES CANADA INC., Vancouver (CA)
Filed by STEMCELL TECHNOLOGIES CANADA INC., Vancouver (CA)
Filed on Feb. 27, 2024, as Appl. No. 18/588,665.
Application 18/588,665 is a continuation of application No. 16/527,177, filed on Jul. 31, 2019, abandoned.
Claims priority of provisional application 62/713,189, filed on Aug. 1, 2018.
Prior Publication US 2024/0207855 A1, Jun. 27, 2024
Int. Cl. B01L 3/00 (2006.01); C12M 1/32 (2006.01)
CPC B01L 3/5085 (2013.01) [C12M 23/12 (2013.01); B01L 2300/0829 (2013.01)] 17 Claims
OG exemplary drawing
 
1. A method for manipulating a biological sample, the method comprising:
a) providing a resilient and foldable silicone-based mat comprising an anti-static additive, the mat having a top planar surface spaced apart from a bottom planar surface and a plurality of recesses arranged in rows and columns and formed in the top planar surface extending toward the bottom planar surface, the recesses having an opening at the top planar surface and bounded by a non-planar and curved or arcuate bottom wall, wherein a width of each of the plurality of recesses is about 0.5 cm and a depth of each recess measured vertically from a center of the opening at the top planar surface to the bottom wall is about 0.1 cm, and wherein any two adjacent recesses in the column or row direction are separated by the same pitch of between about 0.5 and 1 cm;
b) enveloping the biological sample in at least one of the recesses within a polymerizable solution; and
c) recovering the at least one enveloped biological sample from the mat,
wherein the biological sample is an aggregation of cells, and wherein the aggregation of cells comprises an embryoid body, a spheroid, an organoid, an assembloid, a tissue, or a tissue fragment.