US 12,383,902 B2
Low cost microfluidic device for DNA/RNA isolation, purification and amplification using chip based PCR/RT-PCR for biosensing applications
Jaydeep Bhattacharya, New Delhi (IN); Rajkamal Bharti, Moradabad (IN); Asha Ashwathi Madhavan, Vidyanagar (IN); and Ranjita Ghosh Moulick, Asansol (IN)
Assigned to Jaydeep Bhattacharya, New Delhi (IN)
Appl. No. 16/968,416
Filed by Jaydeep Bhattacharya, New Delhi (IN)
PCT Filed Feb. 5, 2019, PCT No. PCT/IN2019/050090
§ 371(c)(1), (2) Date Aug. 7, 2020,
PCT Pub. No. WO2019/155488, PCT Pub. Date Aug. 15, 2019.
Claims priority of application No. 201811004666 (IN), filed on Feb. 7, 2018.
Prior Publication US 2020/0398274 A1, Dec. 24, 2020
Int. Cl. B01L 3/00 (2006.01); B01L 7/00 (2006.01); C12Q 1/6806 (2018.01); C12N 15/10 (2006.01)
CPC B01L 3/502792 (2013.01) [B01L 7/525 (2013.01); C12Q 1/6806 (2013.01); B01L 3/502707 (2013.01); B01L 2300/0681 (2013.01); B01L 2300/0896 (2013.01); B01L 2300/1827 (2013.01); B01L 2400/043 (2013.01); C12N 15/1017 (2013.01)] 14 Claims
OG exemplary drawing
 
1. A system, comprising:
i. a microfluidic device module for isolating nucleic acid, wherein the nucleic acid is DNA, RNA, or both, from a sample comprising a cell lysate, a bacteria-containing fluid, or a virus-containing fluid,
wherein the microfluidic device module comprises an upper chamber, a lower chamber and a septum between the upper chamber and the lower chamber,
wherein the upper chamber has a first input channel for receiving the sample and a second input channel for a lysis buffer,
wherein the septum has nanopores, formed by anodization of aluminum, and is configured to retain and isolate the nucleic acid from the sample as isolated nucleic acid in the upper chamber and to allow protein from the sample to pass through the nanopores and be collected as waste in the lower chamber;
ii. an on-chip polymerase chain reaction (PCR) module configured for receiving a droplet comprising the isolated nucleic acid,
wherein the on-chip PCR module comprises low voltage resistive heaters, having a maximum voltage of 20 V, to create three fixed and different temperature zones for PCR thermal cycling of the isolated nucleic acid during PCR,
wherein the low voltage resistive heaters are placed at the three vertices of an equilateral triangle to create a circular path as the circumcircle of the equilateral triangle; and
iii. a detection unit at the on-chip PCR module for quantifying PCR products.