US 12,382,892 B2
Barley with increased hydrolytic enzyme activity
Christoph Dockter, Copenhagen (DK); Pai Rosager Pedas, Copenhagen (DK); Søren Knudsen, Copenhagen (DK); Ole Olsen, Copenhagen (DK); Lucia Marri, Copenhagen (DK); Katarzyna Krucewicz, Copenhagen (DK); Finn Lok, Copenhagen (DK); Toni Wendt, Copenhagen (DK); Massimiliano Carciofi, Copenhagen (DK); Hanne Thomsen, Copenhagen (DK); and Magnus Rasmussen, Copenhagen (DK)
Assigned to Carlsberg Breweries A/S, Copenhagen V (DK)
Appl. No. 16/958,098
Filed by Carlsberg A/S, Copenhagen (DK)
PCT Filed Dec. 21, 2018, PCT No. PCT/EP2018/086733
§ 371(c)(1), (2) Date Jun. 25, 2020,
PCT Pub. No. WO2019/129739, PCT Pub. Date Jul. 4, 2019.
Claims priority of application No. 17210964 (EP), filed on Dec. 28, 2017.
Prior Publication US 2021/0395762 A1, Dec. 23, 2021
Int. Cl. A01H 6/46 (2018.01); A01H 5/10 (2018.01); A23L 2/38 (2021.01); C07K 14/41 (2006.01); C07K 14/415 (2006.01); C12C 1/02 (2006.01); C12C 1/027 (2006.01); C12G 3/02 (2019.01); C12G 3/021 (2019.01); C12N 9/24 (2006.01); C12N 9/32 (2006.01); C12N 15/82 (2006.01)
CPC A01H 6/4624 (2018.05) [A01H 5/10 (2013.01); A23L 2/382 (2013.01); C07K 14/415 (2013.01); C12C 1/027 (2013.01); C12G 3/021 (2019.02); C12N 9/2422 (2013.01); C12N 15/8243 (2013.01); C12Y 302/01001 (2013.01); C12C 2200/01 (2013.01)] 11 Claims
 
1. A barley plant or a part thereof, wherein said barley plant carries a mutation in the HvHRT gene resulting in a mutant HvHRT gene encoding a mutant HvHRT protein comprising the amino acid sequence of SEQ ID NO: 2 modified by a W431 stop mutation or a W371 stop mutation, and wherein said part of the barley plant is a kernel, embryo, germinated kernel, leaf, stem, root, flower, milled barley plant, or milled barley kernel.