US 12,379,377 B2
Two-layer microfluidic chip with magnetic bead luminescence and detection system
Dong Wang, Guangdong (CN); Yuxia Fan, Guangdong (CN); and Quan Li, Guangdong (CN)
Assigned to SHENZHEN WATMIND MEDICAL CO., LTD., Shenzhen (CN)
Appl. No. 17/623,875
Filed by SHENZHEN WATMIND MEDICAL CO., LTD., Guangdong (CN)
PCT Filed Oct. 10, 2020, PCT No. PCT/CN2020/120091
§ 371(c)(1), (2) Date Dec. 29, 2021,
PCT Pub. No. WO2021/068914, PCT Pub. Date Apr. 15, 2021.
Claims priority of application No. 201910962678.0 (CN), filed on Oct. 11, 2019.
Prior Publication US 2022/0357320 A1, Nov. 10, 2022
Int. Cl. G01N 33/543 (2006.01); G01N 33/533 (2006.01)
CPC G01N 33/54386 (2013.01) [G01N 33/54326 (2013.01)] 15 Claims
OG exemplary drawing
 
1. A two-layer microfluidic chip with magnetic bead luminescence, comprising:
a top plate, comprising a sample addition section, a conjugated ligand storage section, and a sample mixing area, wherein a conjugated ligand is stored in the conjugated ligand storage section, and the sample mixing area is in communication respectively with the sample addition section and the conjugated ligand storage section; and
a bottom plate provided on the top plate, comprising a flow guiding area in communication with the sample mixing area, a magnetic bead coating section in communication with the flow guiding area, a washing area in communication with the magnetic bead coating section, a detection area in communication with the washing area, and a washing fluid storage section in communication with the washing area, wherein the flow guiding area is arranged with a recess that is lower than a bottom wall of the magnetic bead coating section in the height direction, a flow guiding portion fitted to the recess and connecting the magnetic bead coating section, a cut-off groove below a front end of the flow guiding portion, and a barrier portion provided on the flow guiding portion; and a magnetic bead-conjugated ligand is stored in the magnetic bead coating section, and a washing fluid is stored in the washing fluid storage section.