US 12,379,375 B2
Methods for assessing cell surface glycosylation
Paul Ken Kodama, Seattle, WA (US); Tom Kowski, Seattle, WA (US); Mirna Mujacic, Seattle, WA (US); and Kenneth Mayo Prentice, Seattle, WA (US)
Assigned to Juno Therapeutics, Inc., Seattle, WA (US)
Filed by Juno Therapeutics, Inc., Seattle, WA (US)
Filed on Sep. 15, 2023, as Appl. No. 18/468,626.
Application 18/468,626 is a division of application No. 16/604,547, granted, now 11,796,534, previously published as PCT/US2018/027666, filed on Apr. 13, 2018.
Claims priority of provisional application 62/515,515, filed on Jun. 5, 2017.
Claims priority of provisional application 62/485,897, filed on Apr. 14, 2017.
Prior Publication US 2024/0159743 A1, May 16, 2024
Int. Cl. G01N 33/53 (2006.01); C12Q 1/34 (2006.01); G01N 33/50 (2006.01)
CPC G01N 33/5308 (2013.01) [C12Q 1/34 (2013.01); C12Y 305/01052 (2013.01); G01N 33/502 (2013.01); G01N 2400/10 (2013.01); G01N 2570/00 (2013.01)] 18 Claims
OG exemplary drawing
 
1. A method of assaying a cell composition, the method comprising:
(a) (i) assessing the cell surface profile in a sample from a test cell composition comprising a plurality of cells according to a method for assessing cell surface glycans comprising:
(1) incubating the test cell composition comprising a plurality of cells under conditions to release one or more glycans from the surface of cells in the test cell composition, wherein a sample comprising one or more cell surface glycans is generated; and
(2) determining the presence, absence, identity and/or level of glycans present in the sample, thereby assessing the cell surface glycan profile of the sample; and
(a) (ii) comparing the cell surface glycan profile of the sample to the cell surface glycan profile of a reference sample; or
(b) comparing the cell surface glycan profile of a sample with the cell surface profile of a reference sample, wherein cell surface glycan profile of the sample is or has been determined according to a method for assessing cell surface glycans comprising:
(1) incubating a test cell composition comprising a plurality of cells under conditions to release one or more glycans from the surface of cells in the test cell composition, wherein a sample comprising one or more cell surface glycans is generated; and
(2) determining the presence, absence, identity and/or level of glycans present in the sample, thereby assessing the cell surface glycan profile of the sample; and
wherein the cells express a recombinant receptor or the test composition comprises cells expressing a recombinant receptor.