US 12,379,344 B2
Electronic devices for polymer synthesis and assembly
Matthew T. Holden, San Diego, CA (US); Barry Merriman, San Diego, CA (US); Ryan De Ridder, San Diego, CA (US); Kyle T. Gustafson, Glendale, CA (US); and Michael Heller, Geneva, IL (US)
Assigned to Avery Digital Data, Inc., San Diego, CA (US)
Appl. No. 17/909,536
Filed by Avery Digital Data, Inc., San Diego, CA (US)
PCT Filed Jun. 9, 2022, PCT No. PCT/US2022/032894
§ 371(c)(1), (2) Date Sep. 6, 2022,
PCT Pub. No. WO2022/261366, PCT Pub. Date Dec. 15, 2022.
Claims priority of provisional application 63/208,669, filed on Jun. 9, 2021.
Prior Publication US 2024/0011941 A1, Jan. 11, 2024
Int. Cl. G01N 27/414 (2006.01); B01J 19/00 (2006.01); G01N 27/327 (2006.01)
CPC G01N 27/4148 (2013.01) [G01N 27/3274 (2013.01); G01N 27/3277 (2013.01); G01N 27/4145 (2013.01)] 17 Claims
OG exemplary drawing
 
1. A system comprising:
a polymer synthesis and assembly chip comprising:
a complementary metal-oxide semiconductor (CMOS) integrated circuit device comprising:
a plurality of pixels, wherein each pixel of the plurality of pixels includes an electrode;
wherein a first set of one or more pixels of the plurality of pixels is configured to electrochemically synthesize a first oligonucleotide as a de novo construction through electrically controlled localized reactions within a fluid medium; and
wherein a second set of one or more pixels of the plurality of pixels is configured to assemble the first oligonucleotide with a second oligonucleotide into a larger oligonucleotide construct by electrically controlling motion of the first oligonucleotide, selectively, within the fluid medium, either independently or in concert with one or more pixels of the plurality of pixels;
control circuitry capable of applying voltages to the electrode;
a fluidic system capable of programmed delivery of the fluid medium; and
a gasket to confine the fluid medium in proximity to the plurality of pixels.