| CPC G01N 21/3563 (2013.01) [G01N 2201/0636 (2013.01)] | 11 Claims |

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1. A method for spectroscopic imaging, comprising:
(a) illuminating a sample with a short infrared pulse shorter than or equal to the cooling time constant of resolution scale inclusions within the sample to induce a temperature change independent of inclusion size or surface area, wherein the sample has a chemical makeup;
(b) directing one or more probe beams to the sample such that it is incident within the area heated by the infrared pulse, and wherein the one or more probe beams has a shorter wavelength than the infrared pulse; and
(c) measuring light that is reflected, transmitted, re-emitted inelastically, or any combination thereof for the one or more probe beam;
(d) repeating steps (a)-(c) multiple times with varying wavelengths of the infrared pulse and while moving the sample and the one or more probe beams relative to each other to create a multidimensional infrared spectroscopic image of the sample that characterizes the chemical makeup of the sample; and
(e) analyzing the multidimensional infrared spectroscopic image using spectral de-mixing to extract locations and spectra of distinct analytes within the sample.
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