US 12,379,301 B2
Closed-system sorting flow cytometer adapters and methods of use thereof
Wesley Howell, San Jose, CA (US)
Assigned to BECTON, DICKINSON AND COMPANY, Franklin Lakes, NJ (US)
Filed by Becton, Dickinson and Company, Franklin Lakes, NJ (US)
Filed on Feb. 23, 2022, as Appl. No. 17/678,476.
Claims priority of provisional application 63/165,339, filed on Mar. 24, 2021.
Prior Publication US 2022/0326138 A1, Oct. 13, 2022
Int. Cl. G01N 15/1404 (2024.01); G01N 15/10 (2024.01); G01N 15/14 (2024.01); G01N 15/149 (2024.01)
CPC G01N 15/1404 (2013.01) [G01N 15/1459 (2013.01); G01N 2015/1006 (2013.01); G01N 15/149 (2024.01)] 19 Claims
OG exemplary drawing
 
1. A sorting flow cytometer, the sorting flow cytometer comprising:
an illumination source for illuminating a flow cell at an interrogation point;
a detector for receiving light from the interrogation point;
a sort block in liquid-receiving relationship with the flow cell; and
a closed-system sorting flow cytometer adapter in droplet-receiving relationship with the sort block, the adapter comprising:
a sort block coupler operably connected to the sort block;
an external chamber; and
a connector gaseously coupling the sort block to the external chamber, optionally via a sort bucket that is in cellular-receiving relationship with the sort block coupler,
wherein the adaptor is configured to generate a closed positive pressure system within the sorting flow cytometer.