US 12,378,601 B2
Multiplex Y-STR analysis
Christina Bormann Chung, Half Moon Bay, CA (US); Julio Mulero, Carlsbad, CA (US); and Lori Hennessy, San Mateo, CA (US)
Assigned to Life Technologies Corporation, Carlsbad, CA (US)
Filed by LIFE TECHNOLOGIES CORPORATION, Carlsbad, CA (US)
Filed on Feb. 24, 2020, as Appl. No. 16/799,810.
Application 16/799,810 is a division of application No. 14/727,442, filed on Jun. 1, 2015, granted, now 10,597,707.
Application 14/727,442 is a division of application No. 13/828,443, filed on Mar. 14, 2013, abandoned.
Claims priority of provisional application 61/761,152, filed on Feb. 5, 2013.
Claims priority of provisional application 61/720,949, filed on Oct. 31, 2012.
Claims priority of provisional application 61/697,742, filed on Sep. 6, 2012.
Claims priority of provisional application 61/765,323, filed on Feb. 15, 2013.
Prior Publication US 2020/0255892 A1, Aug. 13, 2020
Int. Cl. C12Q 1/68 (2018.01); C12Q 1/6858 (2018.01); C12Q 1/6879 (2018.01); C12Q 1/6888 (2018.01); C12Q 1/686 (2018.01)
CPC C12Q 1/6858 (2013.01) [C12Q 1/6879 (2013.01); C12Q 1/6888 (2013.01); C12Q 1/686 (2013.01); C12Q 2600/156 (2013.01); C12Q 2600/16 (2013.01)] 10 Claims
OG exemplary drawing
 
1. A kit for co-amplification of a set of loci in a DNA sample, the kit comprising a set of amplification primers provided together in the kit, the set of amplification primers comprising primers configured for the amplification of:
Y-STR markers DYS576, DYS389I, DYS460, DYS458, DYS19, DYS456, DYS390, DYS570, DYS437, DYS393, and DYS439,
wherein the kit omits one or more of Y-STR markers DYS385ab, DYS389II, DYS391, DYS392, DYS438, DYS448, or Y-GATA-H4, and
wherein the kit comprises an allelic ladder that contains allelic size standards for known alleles of the Y-STR markers DYS576, DYS389I, DYS460, DYS458, DYS19, DYS456, DYS390, DYS570, DYS437, DYS393, and DYS439,
wherein, for each Y-STR marker, the allelic size standards cover a range from a smallest allele to a largest allele amplified by the set of amplification primers configured to amplify the Y-STR marker,
wherein each range of allelic size standards is separated by an at least two base pair difference from a preceding range of allelic size standards for a first adjacent Y-STR marker and a proceeding range of allelic size standards for a second adjacent Y-STR marker.