	US 12,378,580 B2
	Co-production pathway for 3-HPA and acetyl-CoA derivatives from malonate semialdehyde
Beatriz Leite Magalhaes, Campinas (BR); Paulo Moises Raduan Alexandrino, Campinas (BR); and Felipe Galzerani, Paulínia (BR)
Assigned to Braskem S.A., Sao Paulo (BR)
Filed by Braskem S.A., Sao Paulo (BR)
Filed on Jul. 1, 2022, as Appl. No. 17/856,863.
Application 17/856,863 is a continuation of application No. 16/719,833, filed on Dec. 18, 2019, granted, now 11,377,671.
Claims priority of provisional application 62/781,511, filed on Dec. 18, 2018.
Prior Publication US 2023/0037707 A1, Feb. 9, 2023
Int. Cl. C12P 7/42 (2006.01); C12N 1/16 (2006.01); C12N 1/18 (2006.01); C12R 1/865 (2006.01)

CPC C12P 7/42 (2013.01) [C12N 1/16 (2013.01); C12N 1/185 (2021.05); C12R 2001/865 (2021.05); C12Y 101/01 (2013.01); C12Y 203/01009 (2013.01)]

22 Claims

1. A recombinant microorganism capable of producing acetone and/or isopropanol from a feedstock comprising one or more carbon sources, wherein the recombinant microorganism comprises:

(a) at least one endogenous and/or exogenous nucleic acid molecule encoding an aspartate amino transferase that catalyzes the production of aspartate from oxaloacetate;

(b) at least one exogenous nucleic acid molecule encoding an aspartate decarboxylase that catalyzes the production of β-alanine from aspartate from (a);

(c) at least one endogenous and/or exogenous nucleic acid molecule encoding a β-alanine pyruvate amino transferase and/or a β-alanine transaminase that catalyzes the conversion of β-alanine from (b) to malonate semialdehyde; and

(d) a pathway for the conversion of malonate semialdehyde to acetone.