US 12,378,577 B2
Crispr-CAS10 systems and methods for phage genome editing
Asma Hatoum, Northport, AL (US)
Assigned to The Board of Trustees of The University of Alabama, Tuscaloosa, AL (US)
Filed by The Board of Trustees of The University of Alabama, Tuscaloosa, AL (US)
Filed on Sep. 16, 2022, as Appl. No. 17/932,915.
Application 17/932,915 is a division of application No. 15/910,620, filed on Mar. 2, 2018, granted, now 11,453,892.
Claims priority of provisional application 62/465,929, filed on Mar. 2, 2017.
Prior Publication US 2023/0041099 A1, Feb. 9, 2023
Int. Cl. C12N 15/86 (2006.01); C12N 1/20 (2006.01); C12N 9/12 (2006.01); C12N 9/22 (2006.01); C12N 15/11 (2006.01); C12N 15/74 (2006.01); C12N 15/90 (2006.01); C12R 1/44 (2006.01)
CPC C12N 15/86 (2013.01) [C12N 1/205 (2021.05); C12N 9/1276 (2013.01); C12N 9/22 (2013.01); C12N 15/11 (2013.01); C12N 15/74 (2013.01); C12N 15/902 (2013.01); C12N 2310/20 (2017.05); C12N 2710/00042 (2013.01); C12N 2710/00045 (2013.01); C12N 2795/10121 (2013.01); C12N 2795/10221 (2013.01); C12N 2795/10321 (2013.01); C12R 2001/44 (2021.05)] 7 Claims
OG exemplary drawing
 
1. A phage genome editing system comprising:
a Staphylococcus bacterial cell that can be infected by a phage;
a vector comprising a crRNA that can hybridize to a nucleic acid sequence of the phage;
a vector comprising a CRISPR-Cas10 system, wherein the CRISPR-Cas10 system comprises CRISPR-associated genes csm1/cas10, csm2, csm3, csm4, csm5, csm6, and/or cas6; and
a vector comprising a donor nucleic acid sequence, wherein the donor nucleic acid sequence comprises: a mutated nucleic acid sequence to be introduced into the phage, flanked by two nucleic acid sequences containing regions of homology to the phage genome.