	US 12,378,575 B2
	Chemically-defined baculovirus expression system
Maya Yovcheva, Demascus, MD (US); Jonathan Zmuda, Frederick, MD (US); Sara Barnes, Germantown, MD (US); Chang Choi, San Diego, CA (US); and Kenneth Thompson, Burtonsville, MD (US)
Assigned to Life Technologies Corporation, Carlsbad, CA (US)
Appl. No. 17/046,693
Filed by LIFE TECHNOLOGIES CORPORATION, Carlsbad, CA (US)
PCT Filed Apr. 11, 2019, PCT No. PCT/US2019/027108 § 371(c)(1), (2) Date Oct. 9, 2020, PCT Pub. No. WO2019/200184, PCT Pub. Date Oct. 17, 2019.
Claims priority of provisional application 62/656,868, filed on Apr. 12, 2018.
Prior Publication US 2021/0310024 A1, Oct. 7, 2021
Int. Cl. C12N 15/86 (2006.01); C12N 5/07 (2010.01); C12N 7/00 (2006.01); C12P 21/00 (2006.01)

CPC C12N 15/86 (2013.01) [C12N 5/0601 (2013.01); C12N 7/00 (2013.01); C12P 21/00 (2013.01); C12N 2500/02 (2013.01); C12N 2500/05 (2013.01); C12N 2500/34 (2013.01); C12N 2500/38 (2013.01); C12N 2510/00 (2013.01); C12N 2710/14143 (2013.01); C12N 2710/14151 (2013.01)]

18 Claims

1. A baculovirus expression system comprising:

(a) an insect cell medium, wherein the insect cell medium comprises an energy source selected from maltose, sucrose, glucose, trehalose, fructose, mannose, lactose, galactose, dextrose, or combinations thereof; and

(b) a plurality of Sf9 cells.