	US 12,378,569 B2
	GBWOX3A gene and application thereof in plant tissue culture
Fangfang Fu, Nanjing (CN); Liangjiao Xue, Nanjing (CN); Fuliang Cao, Nanjing (CN); Anqi Zhao, Nanjing (CN); Wei Xu, Taizhou (CN); Pingjun Xu, Qidong (CN); Xiaoming Yang, Nanjing (CN); and Meiling Ming, Nanjing (CN)
Assigned to Nanjing Forestry University, Nanjing (CN)
Filed by Nanjing Forestry University, Nanjing (CN)
Filed on Sep. 5, 2024, as Appl. No. 18/825,366.
Claims priority of application No. 202311195940.6 (CN), filed on Sep. 15, 2023.
Prior Publication US 2025/0092412 A1, Mar. 20, 2025
Int. Cl. C12N 15/82 (2006.01)

CPC C12N 15/8262 (2013.01) [C12N 15/8205 (2013.01)]

1 Claim

1. An expression method of a GbWOX3A gene, comprising:

constructing an overexpression vector of pCAMBIA1302-GbWOX3A, transforming the overexpression vector pCAMBIA1302-GbWOX3A into an Agrobacterium EHA105 competent cell, and transforming a detached leaf of poplar 84K by using an Agrobacterium-mediated method to over-express the GbWOX3A gene in the detached leaf of the poplar 84K for a tissue culture regeneration of the poplar 84K; and

wherein the gene sequence of the GbWOX3A gene is SEQ ID NO: 1, and the amino acid sequence encoded by the GbWOX3A gene is SEQ ID NO: 2.