	US 12,378,567 B2
	Arogenate dehydrogenase polynucleotides, polypeptides and methods of using the same
Hiroshi A. Maeda, Madison, WI (US); Samuel Lopez-Nieves, Madison, WI (US); and Marcos Viana de Oliveira, Madison, WI (US)
Assigned to Wisconsin Alumni Research Foundation, Madison, WI (US)
Filed by Wisconsin Alumni Research Foundation, Madison, WI (US)
Filed on Feb. 16, 2018, as Appl. No. 15/932,337.
Claims priority of provisional application 62/459,798, filed on Feb. 16, 2017.
Prior Publication US 2018/0265880 A1, Sep. 20, 2018
Int. Cl. A01H 5/00 (2018.01); C07H 21/04 (2006.01); C12N 15/00 (2006.01); C12N 15/82 (2006.01); C12P 13/22 (2006.01)

CPC C12N 15/8251 (2013.01) [A01H 5/00 (2013.01); C07H 21/04 (2013.01); C12N 15/00 (2013.01); C12P 13/22 (2013.01); C12Y 103/01043 (2013.01)]

13 Claims

1. A construct comprising a heterologous promoter operably linked to a polynucleotide encoding an arogenate dehydrogenase (ADH) polypeptide selected from:

a) the group consisting of any one of SEQ ID NOS: 1, 5, 7, 10, 12, 13, and 43; and

b) a functional fragment of SEQ ID NO: 43 consisting of amino acids 54-398 of SEQ ID NO: 43;

wherein the polypeptide maintains at least 50% of its ADH activity in the presence of 10 μM tyrosine.