US 12,378,545 B2
Method for nucleic acid depletion
Justin Joseph O'Grady, Norwich (GB); John Richard Wain, Norwich (GB); Solomon Mwaigwisya, Norwich (GB); and Gemma Louise Kay, Norwich (GB)
Assigned to UEA Enterprises Limited, Norwich (GB)
Filed by UEA Enterprises Limited, Norwich (GB)
Filed on Oct. 10, 2022, as Appl. No. 17/962,603.
Application 17/962,603 is a continuation of application No. 16/468,922, granted, now 11,505,793, previously published as PCT/GB2017/053715, filed on Dec. 12, 2017.
Claims priority of application No. 1621271 (GB), filed on Dec. 14, 2016.
Prior Publication US 2023/0183673 A1, Jun. 15, 2023
Int. Cl. C12N 15/10 (2006.01); C12Q 1/6806 (2018.01); C12Q 1/686 (2018.01)
CPC C12N 15/1003 (2013.01) [C12Q 1/6806 (2013.01); C12Q 1/686 (2013.01); C12Y 301/04003 (2013.01)] 16 Claims
OG exemplary drawing
 
1. A method for depleting host nucleic acid in a biological sample while maintaining non-host nucleic acid, said sample having been previously obtained from an animal host, said method comprising the steps of:
(a) adding a phospholipase D (PLD), or an active variant thereof, to said sample to selectively lyse host cells in said biological sample; and
(b) carrying-out a process to physically deplete the host nucleic acid released from the lysed host cells within said biological sample,
wherein the active variant is a variant of a wild-type PLD that has at least 10% of the lytic activity of the wild-type PLD.