	US 12,378,540 B1
	Fusion proteins comprising acid alpha-glucosidase enzymes and methods thereof
Mihalis S. Kariolis, San Mateo, CA (US); Melina Lenser, San Francisco, CA (US); Cathal S. Mahon, San Francisco, CA (US); Hanh Giai Ngo, San Bruno, CA (US); Yashas Rajendra, San Mateo, CA (US); Shrishti Tyagi, Santa Clara, CA (US); and Tianao Yuan, San Mateo, CA (US)
Assigned to DENALI THERAPEUTICS INC., South San Francisco, CA (US)
Filed by DENALI THERAPEUTICS INC., South San Francisco, CA (US)
Filed on Mar. 17, 2025, as Appl. No. 19/081,211.
Application 19/081,211 is a continuation of application No. PCT/US2024/060936, filed on Dec. 19, 2024.
Claims priority of provisional application 63/612,862, filed on Dec. 20, 2023.
Int. Cl. C12N 9/24 (2006.01); A61P 3/00 (2006.01); A61K 38/00 (2006.01)

CPC C12N 9/2402 (2013.01) [A61P 3/00 (2018.01); C12Y 302/0102 (2013.01); A61K 38/00 (2013.01); C07K 2319/30 (2013.01)]

17 Claims

1. A protein comprising

a fusion polypeptide comprising a first Fc polypeptide linked to an acid alpha-glucosidase (GAA) enzyme; and

a second Fc polypeptide; wherein:

(a) the fusion polypeptide comprises SEQ ID NO: 68; and the second Fc polypeptide comprises any one of SEQ ID NOs: 41-42; or

(b) the fusion polypeptide comprises SEQ ID NO: 70; and the second Fc polypeptide comprises any one of SEQ ID NOs:43-44.