US 12,378,539 B2
Evolved integrases and methods of using the same for genome editing
Jesse B. Owens, Honolulu, HI (US); and Brian Hew, Honolulu, HI (US)
Assigned to University of Hawaii, Honolulu, HI (US)
Filed by University of Hawaii, Honolulu, HI (US)
Filed on Oct. 15, 2024, as Appl. No. 18/915,656.
Application 18/915,656 is a continuation of application No. PCT/US2024/035414, filed on Jun. 25, 2024.
Claims priority of provisional application 63/592,945, filed on Oct. 25, 2023.
Claims priority of provisional application 63/510,171, filed on Jun. 26, 2023.
Prior Publication US 2025/0043263 A1, Feb. 6, 2025
Int. Cl. C12N 9/22 (2006.01)
CPC C12N 9/22 (2013.01) 16 Claims
OG exemplary drawing
 
1. A Bxb1 integrase comprising:
an amino acid sequence that is at least 98% identical to the amino acid sequence of SEQ ID NO: 1; and
one or more amino acid mutations at positions selected from L4I, E42K, R57K, R63K, V76I, H111P, V122M, and V187I, relative to SEQ ID NO: 1.