	US 12,378,538 B2
	Phytase mutant
Xiuxiu Wu, Shandong (CN); Yijun Huang, Shandong (CN); Yang Liu, Shandong (CN); Xia Zhang, Shandong (CN); and Sida Cheng, Shandong (CN)
Assigned to QINGDAO VLAND BIOTECH GROUP CO., LTD, Shandong (CN)
Appl. No. 17/431,185
Filed by QINGDAO VLAND BIOTECH GROUP CO., LTD, Shandong (CN)
PCT Filed Feb. 11, 2020, PCT No. PCT/CN2020/074735 § 371(c)(1), (2) Date Aug. 16, 2021, PCT Pub. No. WO2020/168943, PCT Pub. Date Aug. 27, 2020.
Claims priority of application No. 201910119308.0 (CN), filed on Feb. 18, 2019.
Prior Publication US 2022/0154154 A1, May 19, 2022
This patent is subject to a terminal disclaimer.
Int. Cl. C12N 9/16 (2006.01); C12N 15/81 (2006.01)

CPC C12N 9/16 (2013.01) [C12N 15/815 (2013.01); C12Y 301/03 (2013.01)]

8 Claims

1. A phytase mutant, wherein the mutant comprises an amino acid sequence having at least 90% identity with SEQ ID NO: 1, and the mutant comprises an amino acid substitution or a combination selected from the group consisting of:

Q258E,

Q258E/S266P,

V89T/Q258E,

E91Q/Q258E,

Q225Y/Q258E,

Q253Y/Q258E,

V89T/E91Q/Q258E,

V89T/Q225Y/Q258E,

V89T/Q253Y/Q258E,

V89T/Q258E/S266P,

E91Q/Q225Y/Q258E,

E91Q/Q253Y/Q258E,

E91Q/Q258E/S266P,

Q225Y/Q253Y/Q258E,

Q225Y/Q258E/S266P,

V89T/E91Q/Q225Y/Q258E,

V89T/E91Q/Q253Y/Q258E,

V89T/E91Q/Q258E/S266P,

E91Q/Q225Y/Q253Y/Q258E,

E91Q/Q225Y/Q258E/S266P,

V89T/Q225Y/Q253Y/Q258E,

V89T/Q225Y/Q258E/S266P,

E91Q/Q225Y/Q253Y/Q258E,

E91Q/Q225Y/Q258E/S266P,

E91Q/Q253Y/Q258E/S266P,

V89T/Q253Y/Q258E/S266P,

V89T/E91Q/Q225Y/Q253Y/Q258E,

V89T/Q225Y/Q253Y/Q258E/S266P,

V89T/E91Q/Q253Y/Q258E/S266P,

V89T/E91Q/Q225Y/Q258E/S266P,

E91Q/Q225Y/Q253Y/Q258E/S266P,

V89T/E91Q/Q225Y/Q253Y/Q258E/S266P,

S266P,

V89T/S266P,

E91Q/S266P,

Q225Y/S266P,

Q253Y/S266P,

V89T/E91Q/S266P,

V89T/Q225Y/S266P,

V89T/Q253Y/S266P,

E91Q/Q225Y/S266P,

E91Q/Q253Y/S266P,

Q225Y/Q253Y/S266P,

V89T/E91Q/Q225Y/S266P,

V89T/E91Q/Q253Y/S266P,

V89T/Q225Y/Q253Y/S266P,

V89T/E91Q/Q225Y/Q253Y/S266P,

V89T/E91Q,

V89T/Q253Y,

E91Q/Q253Y,

V89T/E91Q/Q253Y,

V89T/E91Q/Q225Y,

V89T/Q225Y/Q253Y,

E91Q/Q225Y/Q253Y,

V89T/E91Q/Q225Y/Q253Y,

V89T/E91Q/A202P/Q253Y,

V89T/E91Q/L213F/Q253Y,

V89T/E91Q/L213W/Q253Y,

V89T/E91Q/W243V/Q253Y,

V89T/E91Q/W243L/Q253Y,

D69F/V89T/E91Q/Q253Y,

D69Q/V89T/E91Q/Q253Y,

V89T/E91Q/T111P/Q253Y,

V89T/E91Q/T238R/Q253Y,

A36P/V89T/E91Q/Q253Y,

V89T/E91Q/A202P/L213F/Q253Y,

V89T/E91Q/A202P/L213W/Q253Y,

V89T/E91Q/A202P/W243V/Q253Y,

V89T/E91Q/A202P/W243L/Q253Y,

V89T/E91Q/L213F/W243V/Q253Y,

V89T/E91Q/L213W/W243L/Q253Y,

V89T/E91Q/L213F/W243L/Q253Y,

V89T/E91Q/L213W/W243V/Q253Y,

V89T/E91Q/A202P/L213F/W243V/Q253Y,

V89T/E91Q/A202P/L213W/W243L/Q253Y,

V89T/E91Q/A202P/L213F/W243L/Q253Y,

V89T/E91Q/A202P/L213W/W243V/Q253Y,

V89T/E91Q/A202P/Q225Y/Q253Y,

V89T/E91Q/L213F/Q225Y/Q253Y,

V89T/E91Q/L213W/Q225Y/Q253Y,

V89T/E91Q/W243V/Q225Y/Q253Y,

V89T/E91Q/W243L/Q225Y/Q253Y,

D69F/V89T/E91Q/Q225Y/Q253Y,

D69Q/V89T/E91Q/Q225Y/Q253Y,

V89T/E91Q/T111P/Q225Y/Q253Y,

V89T/E91Q/T238R/Q225Y/Q253Y,

A36P/V89T/E91Q/Q225Y/Q253Y,

V89T/E91Q/A202P/Q225Y/Q253Y/Q258E/S266P,

V89T/E91Q/L213F/Q225Y/Q253Y/Q258E/S266P,

V89T/E91Q/L213W/Q225Y/Q253Y/Q258E/S266P,

V89T/E91Q/W243V/Q225Y/Q253Y/Q258E/S266P,

V89T/E91Q/W243L/Q225Y/Q253Y/Q258E/S266P,

D69F/V89T/E91Q/Q225Y/Q253Y/Q258E/S266P,

D69Q/V89T/E91Q/Q225Y/Q253Y/Q258E/S266P,

V89T/E91Q/T111P/Q225Y/Q253Y/Q258E/S266P,

V89T/E91Q/T238R/Q225Y/Q253Y/Q258E/S266P,

A36P/V89T/E91Q/Q225Y/Q253Y/Q258E/S266P,

V89T/E91Q/A202P/L213F/Q225Y/Q253Y/Q258E/S266P,

V89T/E91Q/A202P/L213W/Q225Y/Q253Y/Q258E/S266P,

V89T/E91Q/A202P/Q225Y/W243V/Q253Y/Q258E/S266P,

V89T/E91Q/A202P/Q225Y/W243L/Q253Y/Q258E/S266P,

V89T/E91Q/L213F/Q225Y/W243V/Q253Y/Q258E/S266P,

V89T/E91Q/L213W/Q225Y/W243L/Q253Y/Q258E/S266P,

V89T/E91Q/L213F/Q225Y/W243L/Q253Y/Q258E/S266P,

V89T/E91Q/L213W/Q225Y/W243V/Q253Y/Q258E/S266P,

V89T/E91Q/A202P/L213F/Q225Y/W243V/Q253Y/Q258E/S266P,

V89T/E91Q/A202P/L213W/Q225Y/W243L/Q253Y/Q258E/S266P,

V89T/E91Q/A202P/L213F/Q225Y/W243L/Q253Y/Q258E/S266P,

V89T/E91Q/A202P/L213W/Q225Y/W243V/Q253Y/Q258E/S266P,

A202P,

L213F,

L213W,

W243V,

W243L,

D69Q,

D69F,

T111P,

T238R,

A36P,

A202P/L213F,

A202P/L213W,

A202P/W243L,

A202P/W243V,

A202P/L213F/W243L,

A202P/L213F/W243V,

A202P/L213W/W243V, and

A202P/L213W/W243L.