Engineered imine reductases and methods for the reductive amination of ketone and amine compounds
Oscar Alvizo, Fremont, CA (US); Melissa Ann Mayo, Foster City, CA (US); and Jeffrey C. Moore, Westfield, NJ (US)
Assigned to Codexis, Inc., Redwood City, CA (US)
Filed by Codexis, Inc., Redwood City, CA (US)
Filed on Sep. 21, 2023, as Appl. No. 18/471,524.
Application 16/443,250 is a division of application No. 15/519,949, granted, now 10,370,648, issued on Aug. 6, 2019, previously published as PCT/US2015/062300, filed on Nov. 24, 2015.
Application 18/471,524 is a continuation of application No. 17/739,028, filed on May 6, 2022, granted, now 11,802,274.
Application 17/739,028 is a continuation of application No. 17/010,709, filed on Sep. 2, 2020, granted, now 11,352,609, issued on Jun. 7, 2022.
Application 17/010,709 is a continuation of application No. 16/443,250, filed on Jun. 17, 2019, granted, now 10,793,836, issued on Oct. 6, 2020.
Claims priority of provisional application 62/084,037, filed on Nov. 25, 2014.
Prior Publication US 2024/0132856 A1, Apr. 25, 2024 Prior Publication US 2024/0228982 A9, Jul. 11, 2024
1. An engineered polypeptide comprising an amino acid sequence with at least 80% sequence identity to the amino acid sequence of SEQ ID NO: 8 and an amino acid residue other than lysine at the position corresponding to position X168 of SEQ ID NO: 8, wherein the engineered polypeptide has improved imine reductase activity as compared to SEQ ID NO: 8.