US 12,378,528 B2
Systems and methods for growth of intestinal cells in microfluidic devices
S. Jordan Kerns, Reading, MA (US); Norman Wen, West Roxbury, MA (US); Carol Lucchesi, Westwood, MA (US); Christopher David Hinojosa, Malden, MA (US); Jacob Fraser, Somerville, MA (US); Jefferson Puerta, Malden, MA (US); Geraldine Hamilton, Boston, MA (US); Robert Barrett, Los Angeles, CA (US); Clive Svendsen, Pacific Palisades, CA (US); Daniel Levner, Brookline, MA (US); Stephen R. Targan, Santa Monica, CA (US); Michael Workman, Santa Monica, CA (US); Dhruv Sareen, Porter Ranch, CA (US); Uthra Rajamani, Los Angeles, CA (US); and Magdalena Kasendra, Boston, MA (US)
Assigned to EMULATE, INC., Boston, MA (US); and CEDARS-SINAI MEDICAL CENTER, Los Angeles, CA (US)
Filed by EMULATE, INC., Boston, MA (US); and CEDARS-SINAI MEDICAL CENTER, Los Angeles, CA (US)
Filed on Apr. 4, 2024, as Appl. No. 18/626,855.
Application 18/626,855 is a continuation of application No. 17/678,485, filed on Feb. 23, 2022, granted, now 11,952,592.
Application 17/678,485 is a continuation of application No. 16/051,004, filed on Jul. 31, 2018, granted, now 11,326,149, issued on May 10, 2022.
Application 16/051,004 is a continuation of application No. PCT/US2017/016079, filed on Feb. 1, 2017.
Claims priority of provisional application 62/437,314, filed on Dec. 21, 2016.
Claims priority of provisional application 62/354,040, filed on Jun. 23, 2016.
Claims priority of provisional application 62/332,849, filed on May 6, 2016.
Claims priority of provisional application 62/289,521, filed on Feb. 1, 2016.
Prior Publication US 2024/0254449 A1, Aug. 1, 2024
Int. Cl. C12N 5/071 (2010.01); C12M 3/00 (2006.01); C12M 3/06 (2006.01); C12N 5/074 (2010.01); C12N 5/079 (2010.01); G01N 33/50 (2006.01)
CPC C12N 5/0679 (2013.01) [C12M 21/08 (2013.01); C12M 23/16 (2013.01); C12N 5/0618 (2013.01); C12N 5/0696 (2013.01); G01N 33/5005 (2013.01); C12N 2501/11 (2013.01); C12N 2501/119 (2013.01); C12N 2501/13 (2013.01); C12N 2501/155 (2013.01); C12N 2501/16 (2013.01); C12N 2501/24 (2013.01); C12N 2501/25 (2013.01); C12N 2501/415 (2013.01); C12N 2501/998 (2013.01); C12N 2501/999 (2013.01); C12N 2506/45 (2013.01); C12N 2535/00 (2013.01)] 4 Claims
OG exemplary drawing
 
1. A method of culturing cells, comprising: a) providing i) induced pluripotent stem cell-derived (iPSC-derived) foregut organoids and ii) a fluidic device comprising a membrane, said membrane comprising a top surface and a bottom surface; b) seeding said iPSC-derived foregut organoids onto said top or bottom surface in the presence of epidermal growth factor (EGF) so as to create seeded organoids, said EGF at an initial concentration in media; and c) decreasing said initial EGF concentration in said media gradually over days to induce differentiation and maturation of foregut epithelium.