US 12,378,516 B2
Passage timing calculation device, passage timing calculation method, and recording medium for recording program
Tomoro Dan, Yokohama (JP); Hiroaki Kii, Fujisawa (JP); Takayuki Uozumi, Machida (JP); Yoshiko Sato, Kyoto (JP); and Shinsuke Yoshida, Kyoto (JP)
Assigned to NIKON CORPORATION, Tokyo (JP); and KYOTO UNIVERSITY, Kyoto (JP)
Filed by NIKON CORPORATION, Tokyo (JP); and KYOTO UNIVERSITY, Kyoto (JP)
Filed on Jul. 3, 2024, as Appl. No. 18/763,141.
Application 18/763,141 is a continuation of application No. 17/224,591, filed on Apr. 7, 2021, granted, now 12,054,701.
Application 17/224,591 is a continuation of application No. PCT/JP2019/038926, filed on Oct. 2, 2019.
Claims priority of application No. 2018-193875 (JP), filed on Oct. 12, 2018.
Prior Publication US 2024/0352397 A1, Oct. 24, 2024
This patent is subject to a terminal disclaimer.
Int. Cl. C12M 1/34 (2006.01); C12M 1/36 (2006.01); C12N 5/074 (2010.01); C12M 1/00 (2006.01); C12M 1/26 (2006.01)
CPC C12M 41/46 (2013.01) [C12M 41/36 (2013.01); C12M 41/48 (2013.01); C12N 5/0696 (2013.01); C12M 31/10 (2013.01); C12M 33/00 (2013.01)] 15 Claims
OG exemplary drawing
 
1. A passage timing calculation device comprising:
a processor;
a memory encoded with instructions executed by the processor, the instructions causing the processor to perform operations comprising:
extracting a colony region of pluripotent stem cells from a plurality of images of pluripotent stem cells captured over time in a process of culturing the pluripotent stem cells;
extracting, as an extraction target region, a high-contrast region in which contrast in a gap between the plurality of pluripotent stem cells becomes higher during a transition state between a colony immature state and a mature state, the high-contrast region being a region in which a striated pattern is present in the plurality of images due to the higher contrast;
calculating a time change in a ratio of an area occupied by the extraction target region to a colony region from the plurality of images of pluripotent stem cells captured over time; and
calculating a passage timing of the pluripotent stem cells based on the time change.