US 12,378,512 B2
Culture apparatus for drug discovery research
Koichi Nakayama, Saga (JP)
Assigned to SAGA UNIVERSITY, Saga (JP)
Appl. No. 17/438,614
Filed by SAGA UNIVERSITY, Saga (JP)
PCT Filed Mar. 13, 2020, PCT No. PCT/JP2020/012570
§ 371(c)(1), (2) Date Sep. 13, 2021,
PCT Pub. No. WO2020/189794, PCT Pub. Date Sep. 24, 2020.
Claims priority of application No. 2019-048228 (JP), filed on Mar. 15, 2019.
Prior Publication US 2022/0213424 A1, Jul. 7, 2022
Int. Cl. C12M 1/12 (2006.01); B33Y 80/00 (2015.01); C12N 5/071 (2010.01); G01N 33/50 (2006.01)
CPC C12M 25/14 (2013.01) [B33Y 80/00 (2014.12); C12N 5/0671 (2013.01); G01N 33/5014 (2013.01); G01N 33/5038 (2013.01); C12N 2513/00 (2013.01)] 11 Claims
OG exemplary drawing
 
1. A cell culture apparatus having a cell-holding container and needle-shaped bodies arranged on a substrate, wherein
the center of the bottom surface of the cell-holding container is formed in a protruding shape, the area between the center and a side wall is formed in a recessed shape, and through-holes through which the needle-shaped bodies penetrate are established on the bottom surface of the recessed shape area,
the needle-shaped bodies are arranged in correspondence with the positions of the through-holes, and
the needle-shaped bodies on the substrate are arranged, such that the tip-side portion of each of the needle-shaped bodies penetrates through the corresponding through-hole from the bottom surface side or upper surface side of the cell-holding container.