US 12,378,300 B2
Elimination of CD19-positive lymphoid malignancies by CD19-car expressing NK cells
Hans G. Klingemann, San Diego, CA (US); Laurent H. Boissel, San Diego, CA (US); and Patrick Soon-Shiong, San Diego, CA (US)
Assigned to ImmunityBio, Inc., San Diego, CA (US)
Filed by ImmunityBio, Inc., San Diego, CA (US)
Filed on Jul. 21, 2020, as Appl. No. 16/934,606.
Application 16/934,606 is a division of application No. 16/529,251, filed on Aug. 1, 2019, granted, now 10,765,701.
Claims priority of provisional application 62/753,719, filed on Oct. 31, 2018.
Prior Publication US 2020/0376033 A1, Dec. 3, 2020
Int. Cl. C07K 14/735 (2006.01); A61K 9/00 (2006.01); A61K 40/15 (2025.01); A61K 40/31 (2025.01); A61K 40/42 (2025.01); A61P 35/00 (2006.01); A61P 35/04 (2006.01); C07K 14/54 (2006.01); C07K 14/55 (2006.01); C07K 14/725 (2006.01); C07K 16/28 (2006.01); C12N 5/0783 (2010.01); A61K 38/00 (2006.01); A61K 39/00 (2006.01)
CPC C07K 14/70535 (2013.01) [A61K 9/0019 (2013.01); A61K 40/15 (2025.01); A61K 40/31 (2025.01); A61K 40/4211 (2025.01); A61P 35/00 (2018.01); A61P 35/04 (2018.01); C07K 14/5443 (2013.01); C07K 14/55 (2013.01); C07K 14/7051 (2013.01); C07K 16/2803 (2013.01); C12N 5/0646 (2013.01); A61K 38/00 (2013.01); A61K 2039/54 (2013.01); A61K 2239/31 (2023.05); A61K 2239/48 (2023.05); C07K 2317/622 (2013.01); C07K 2317/732 (2013.01); C07K 2317/76 (2013.01); C07K 2319/02 (2013.01); C07K 2319/30 (2013.01); C07K 2319/33 (2013.01)] 19 Claims
 
1. A recombinant nucleic acid comprising a sequence that encodes an anti-cluster of differentiation 19 (CD19) chimeric antigen receptor (CAR), wherein the anti-CD19 CAR comprises an antigen binding domain comprising a CD19-specific single chain variable fragment (scfv), wherein the C-terminus of the scfv is fused via a peptide linker to the N-terminus of a hinge domain, wherein an intracellular signaling domain comprises the hinge domain at its N-terminus, a transmembrane domain, and an Fc∈RIγ cytoplasmic signaling domain at its C-terminus, and wherein the intracellular signaling domain has the amino acid sequence of amino acids 274-407 of SEQ ID NO: 12.