	US 12,377,423 B2
	Centrifuge system for separating cells in suspension
Stephen B. Kessler, Princeton, MA (US); and T. David Marro, Magnolia, MA (US)
Assigned to PNEUMATIC SCALE CORPORATION, Cuyahoga Falls, OH (US)
Filed by Pneumatic Scale Corporation, Cuyahoga Falls, OH (US)
Filed on Dec. 26, 2023, as Appl. No. 18/396,084.
Application 18/396,084 is a continuation of application No. 17/587,691, filed on Jan. 28, 2022, granted, now 11,878,311.
Application 17/587,691 is a continuation of application No. 17/323,154, filed on May 18, 2021.
Application 17/323,154 is a continuation of application No. 16/433,180, filed on Jun. 6, 2019, granted, now 11,065,629, issued on Jul. 20, 2021.
Application 16/433,180 is a continuation in part of application No. 16/003,659, filed on Jun. 8, 2018, granted, now 11,033,911, issued on Jun. 15, 2021.
Application 16/433,180 is a continuation in part of application No. 15/886,382, filed on Feb. 1, 2018, granted, now 10,384,216, issued on Aug. 20, 2019.
Application 16/003,659 is a continuation of application No. 15/886,382, filed on Feb. 1, 2018, granted, now 10,384,216, issued on Aug. 20, 2019.
Application 15/886,382 is a continuation in part of application No. 14/698,995, filed on Apr. 29, 2015, abandoned.
Application 14/698,995 is a continuation of application No. 13/684,051, filed on Nov. 21, 2012, granted, now 9,222,067, issued on Dec. 29, 2015.
Claims priority of provisional application 62/682,376, filed on Jun. 8, 2018.
Claims priority of provisional application 61/562,438, filed on Nov. 21, 2011.
Prior Publication US 2024/0131529 A1, Apr. 25, 2024
Int. Cl. B04B 11/04 (2006.01); B04B 5/04 (2006.01); B04B 11/02 (2006.01); B04B 11/08 (2006.01)

CPC B04B 11/04 (2013.01) [B04B 11/02 (2013.01); B04B 11/082 (2013.01); B04B 2005/0464 (2013.01)]

28 Claims

1. Apparatus comprising:

a multi-use rotatable centrifuge bowl, wherein the bowl is rotatable about a vertically extending axis and includes an axially centered cavity that is radially bounded by a bowl cavity wall,

a single use structure, wherein the single use structure is configured to be removably positioned in the axially centered cavity in engaged relation with the bowl,

wherein the single use structure bounds an interior area in which cells in a cell culture are separated through centrifugal force as a result of rotation of the single use structure, into cell centrate and cell concentrate,

wherein in an operative position the single use structure includes

an upper disc shape portion,

an annular outer wall, wherein the annular outer wall externally is at least partially in radially abutting relation with the bowl cavity wall,

is in operative fluid tight relation with the upper disc shape portion adjacent to an upper end of the annular outer wall,

internally bounds a separation chamber in which the cell centrate and the cell concentrate are separated,

wherein at least a portion of the separation chamber has a vertically truncated cone shape with a smaller inside radius adjacent to a lower end of the annular outer wall that is axially lower than the upper end,

at least one vertically extending axially centered fluid tight tube, wherein the at least one axially centered fluid tight tube

extends through the upper disc shape portion,

is in fluid connection with the separation chamber,

is operative to at least one of

deliver cell culture into the interior area of the single use structure,

deliver cell concentrate out of the interior area of the single use structure, and

deliver cell centrate out of the interior area of the single use structure.