US 12,377,412 B2
Sample analysis device
Malte Kuhnemund, Solna (SE); Ivan Hernandez-Neuta, Stockholm (SE); and Felix Neumann, Stockholm (SE)
Assigned to COUNTAGEN AB, Stockholm (SE)
Appl. No. 17/602,161
Filed by COUNTAGEN AB, Stockholm (SE)
PCT Filed Apr. 16, 2020, PCT No. PCT/EP2020/060771
§ 371(c)(1), (2) Date Oct. 7, 2021,
PCT Pub. No. WO2020/212531, PCT Pub. Date Oct. 22, 2020.
Claims priority of application No. 1930131-6 (SE), filed on Apr. 16, 2019.
Prior Publication US 2022/0193662 A1, Jun. 23, 2022
Int. Cl. B01L 3/00 (2006.01); C12N 15/10 (2006.01)
CPC B01L 3/50255 (2013.01) [C12N 15/1017 (2013.01); B01L 2200/0668 (2013.01); B01L 2300/0663 (2013.01); B01L 2300/0681 (2013.01); B01L 2300/069 (2013.01); B01L 2300/0829 (2013.01); B01L 2300/0887 (2013.01); B01L 2300/165 (2013.01); B01L 2400/0406 (2013.01)] 7 Claims
 
1. A sample analysis device comprising:
a sample receiving well or multiple sample receiving wells configured to receive a liquid sample comprising rolling circle amplification (RCA) products (RCPs), the sample receiving well having an area that is no larger than a field of detection area of an optical sensing device, and wherein the area of the sample receiving well has a diameter of 0.5 μm to 9 mm;
a filter membrane or a stack of multiple filter membranes permeable for liquid of the liquid sample but substantially impermeable for RCPs in the liquid sample, wherein the sample receiving well is defined in the filter membrane or is in liquid connection with the filter membrane;
a liquid-impermeable layer connected to the filter membrane and comprising a through hole, the through hole defining the area of the sample receiving well; and
an absorption layer or stack of multiple absorption layers in liquid connection with the filter membrane and configured to suck liquid from the liquid sample received in the sample receiving well through the filter membrane by capillary force; and
a spacer layer sandwiched between the liquid-impermeable layer and the absorption layer, wherein the spacer layer comprises a through hole configured to house the filter membrane and wherein the spacer layer is liquid-impermeable.