US 12,377,139 B2
Ehrlichia ruminantium immunogenic compositions and methods of using thereof
Bonto Faburay, Manhattan, KS (US); and Jodi McGill, Manhattan, KS (US)
Assigned to Kansas State University Research Foundation, Manhattan, KS (US)
Appl. No. 16/630,212
Filed by Kansas State University Research Foundation, Manhattan, KS (US)
PCT Filed Jul. 12, 2018, PCT No. PCT/US2018/041863
§ 371(c)(1), (2) Date Jan. 10, 2020,
PCT Pub. No. WO2019/014469, PCT Pub. Date Jan. 17, 2019.
Claims priority of provisional application 62/531,697, filed on Jul. 12, 2017.
Prior Publication US 2020/0129604 A1, Apr. 30, 2020
Int. Cl. A61K 39/02 (2006.01); A61K 9/00 (2006.01); A61K 39/39 (2006.01); A61P 31/04 (2006.01); C07K 14/29 (2006.01); A61K 39/00 (2006.01)
CPC A61K 39/0233 (2013.01) [A61K 9/0019 (2013.01); A61K 39/39 (2013.01); A61P 31/04 (2018.01); C07K 14/29 (2013.01); A61K 2039/54 (2013.01); A61K 2039/545 (2013.01); A61K 2039/55566 (2013.01); C07K 2319/02 (2013.01)] 12 Claims
 
1. A composition comprising a recombinant antigenic protein subunit from a tick-borne pathogen selected from the group consisting of Ehrlichia ruminantium, Ehrlichia chaffeensis, Ehrlichia canis, Ehrlichia ewingii, Ehrlichia muris, Anaplasma phagocytophilum, Anaplasma platys, Anaplasma marginale, and any combination thereof, wherein the subunit has been glycosylated, and further comprising the MAP1 signal peptide from Ehrlichia ruminantium, the adjuvant montanide ISA25, and an additional element selected from the group consisting of at least one additional antigen from a pathogen other than one of said tick-borne pathogens, a preservative, a stabilizer, a color, a flavor, and any combination thereof, wherein the recombinant antigenic protein subunit has been glycosylated from expression through a mammalian or eukaryotic system, and wherein said recombinant antigenic protein subunit has at least 90% sequence homology with a sequence selected from the group consisting of SEQ ID No. 3, SEQ ID No. 4, SEQ ID No. 6, SEQ ID No. 7, SEQ ID No. 12, SEQ ID No. 13, and any combination thereof.