| CPC A61B 5/1486 (2013.01) [A61B 5/14532 (2013.01); A61B 5/4839 (2013.01); A61B 2562/125 (2013.01)] | 9 Claims |

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1. A diabetes biosensor, wherein the biosensor comprises:
a porous polymer membrane with a thickness of 100 μm to 1 mm;
two sides of the porous polymer membrane are provided with impermeable membranes, wherein the porous polymer membrane is made of a mesoporous polymer, wherein the mesoporous polymer is a mixture of polyglycidyl methacrylate and polyethylene glycol, or polyvinyl alcohol;
a first impermeable membrane on one side is provided with a first electrode, a second impermeable membrane on the other side is provided with a second electrode;
a surface of the first electrode is provided with an enzyme layer, and a surface of the enzyme layer is provided with an enzyme protective layer;
an immobilization agent layer is provided between the enzyme protective layer and the enzyme layer, wherein an immobilization agent on the enzyme layer is chitosan; and
the first and second impermeable membranes are selected from a commercial membrane or self-made.
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