US 12,376,609 B2
Thermolabile pigments for meat substitutes derived by mutation of the pigment of coral Echinopora forskaliana
Hans H. Liao, Plymouth, MN (US); Tristan Lipkie, Maple Grove, MN (US); and Chris K. Miller, Andover, MN (US)
Assigned to Cargill, Incorporated, Wayzata, MN (US)
Filed by Cargill, Incorporated, Wayzata, MN (US)
Filed on Sep. 14, 2023, as Appl. No. 18/466,983.
Application 18/466,983 is a continuation of application No. PCT/US2022/020119, filed on Mar. 14, 2022.
Claims priority of provisional application 63/161,158, filed on Mar. 15, 2021.
Prior Publication US 2024/0032569 A1, Feb. 1, 2024
Int. Cl. A23L 5/43 (2016.01); A23J 3/16 (2006.01); A23J 3/18 (2006.01); A23J 3/22 (2006.01); A23L 5/10 (2016.01); A23L 5/42 (2016.01); C07K 14/435 (2006.01); C09B 61/00 (2006.01)
CPC A23L 5/43 (2016.08) [A23J 3/16 (2013.01); A23J 3/18 (2013.01); A23J 3/227 (2013.01); A23L 5/10 (2016.08); C07K 14/43595 (2013.01); C09B 61/00 (2013.01); A23L 5/42 (2016.08)] 29 Claims
 
1. A thermolabile red chromogenic protein (RCP) polypeptide comprising a sequence at least 80% identical to SEQ ID NO: 1 and comprising an XYG chromophore tripeptide and a mutation at a position selected from the group consisting of phenylalanine (F) 68, threonine (T) 102, aspartate (D) 119, D130, alanine (A) 139, F163, F206, A214, and combinations thereof relative to SEQ ID NO:1, wherein the absorbance of the thermolabile RCP polypeptide at 580 nm after heating at 80° C. for 20 minutes is decreased by at least 20% relative to the absorbance at 580 nm prior to heating.