US 12,372,520 B2
Method, device and system for determining the concentration of analytes in a sample
Mohiudeen Azhar, Bangalore (IN); Kalpesh Mehta, Singapore (SG); Ragavendar Ms, Flanders, NJ (US); Sudipa Galgalkar, Bangalore (IN); and Ishita Chakraborty, Bangalore (IN)
Assigned to Siemens Healthcare Diagnostics Inc., Tarrytown, NY (US)
Appl. No. 17/264,529
Filed by Siemens Healthcare Diagnostics Inc., Tarrytown, NY (US)
PCT Filed Jul. 31, 2019, PCT No. PCT/US2019/044307
§ 371(c)(1), (2) Date Jan. 29, 2021,
PCT Pub. No. WO2020/033194, PCT Pub. Date Feb. 13, 2020.
Claims priority of provisional application 62/715,026, filed on Aug. 6, 2018.
Prior Publication US 2021/0293803 A1, Sep. 23, 2021
This patent is subject to a terminal disclaimer.
Int. Cl. G01N 33/72 (2006.01); G01N 21/31 (2006.01); G01N 33/543 (2006.01)
CPC G01N 33/54373 (2013.01) [G01N 21/31 (2013.01); G01N 33/72 (2013.01); G01N 2021/3133 (2013.01)] 6 Claims
OG exemplary drawing
 
1. A method of determining a free hemoglobin concentration, a bilirubin concentration, and a lipid concentration in a whole blood sample, the method comprising:
introducing the whole blood sample through a microfluidic channel;
generating a cell-free plasma layer in the microfluidic channel using acoustophoresis;
illuminating the cell-free plasma layer with light at three wavelengths or wavelength ranges, wherein a first wavelength/wavelength range is in a range of from 400 nm to 420 nm, a second wavelength/wavelength range is in a range of from 440 nm to 460 nm, and a third wavelength/wavelength range is in a range of from 520 nm to 650 nm;
obtaining a first monochromatic image of the illuminated cell-free plasma layer at the first wavelength/wavelength range, obtaining a second monochromatic image of the illuminated cell-free plasma layer at the second wavelength/wavelength range, and obtaining a third monochromatic image of the illuminated cell-free plasma layer at the third wavelength/wavelength range; and
analyzing the first, second, and third images to determine the concentration of free hemoglobin based on the first image, the concentration of bilirubin based on the second image, and the concentration of lipid based on the third image, and wherein analyzing the first, second, and third images comprises:
detecting a cell-free plasma layer in the first, second, and third images, wherein the detection comprises defining a threshold of intensity value of pixels associated with the cell-free plasma layer and detecting the cell-free plasma layer in each image based on the threshold;
determining an optical density of the plasma at the first wavelength/wavelength range for the first image, the second wavelength/wavelength range for the second image, and the third wavelength/wavelength range for the third image;
identifying an absorption coefficient for free hemoglobin for the first image, bilirubin for the second image, and lipid for the third image; and
determining the concentration of free hemoglobin based on the optical density and the absorption coefficient for the first image;
determining the concentration of bilirubin based on the optical density and the absorption coefficient for the second image; and
determining the concentration of lipid based on the optical density and the absorption coefficient for the third image.