US 12,372,502 B2
Methods for chromatography resin slurry determination
Nathan Mao, Tarrytown, NY (US); Eric Shierly, Tarrytown, NY (US); Joseph Gallea, Tarrytown, NY (US); Raymond Feng, Tarrytown, NY (US); Tikiri Dissanayake, Tarrytown, NY (US); Bernhard Schilling, Tarrytown, NY (US); and Scott Carver, Tarrytown, NY (US)
Assigned to Regeneron Pharmaceuticals, Inc., Tarrytown, NY (US)
Filed by Regeneron Pharmaceuticals, Inc., Tarrytown, NY (US)
Filed on Oct. 15, 2018, as Appl. No. 16/160,179.
Claims priority of provisional application 62/573,393, filed on Oct. 17, 2017.
Prior Publication US 2019/0113485 A1, Apr. 18, 2019
Int. Cl. G01N 30/56 (2006.01); B01D 15/20 (2006.01)
CPC G01N 30/56 (2013.01) [B01D 15/206 (2013.01); G01N 2030/565 (2013.01)] 19 Claims
OG exemplary drawing
 
1. A method of determining a slurry concentration, comprising:
adding a slurry comprising a resin to a first chromatography column;
pumping a volume of liquid through the first chromatography column at a flow rate;
controlling the flow rate to match a consolidation rate employed by a second chromatography column to form a consolidated resin in the first chromatography column, wherein the volume of liquid is pumped through the first chromatography column by an automated pump system, wherein the automated pump system is programed to cycle on and off for a predetermined period of time or for multiple periods of time;
upon completion of the pumping step, measuring a bed height of the consolidated resin in the first chromatography column;
determining a slurry concentration in the first chromatography column; and
packing the second chromatography column with the determined slurry concentration in the first chromatography column, wherein the second chromatography column has a packing factor within 5% of an ideal packing factor.