	US 12,371,726 B2
	Process to produce mono-rhamnolipids
Natalia Eliza Iyke Domeradzka, Liverpool (GB); Dietmar Andreas Lang, Liverpool (GB); Neil James Parry, Tarporley (GB); and Mark Lawrence Thompson, Ellesmere Port (GB)
Assigned to Conopco, Inc., Englewood Cliffs (GB)
Appl. No. 18/286,782
Filed by Conopco, Inc., Englewood Cliffs, NJ (US)
PCT Filed Apr. 25, 2022, PCT No. PCT/EP2022/060848 § 371(c)(1), (2) Date Oct. 13, 2023, PCT Pub. No. WO2022/229069, PCT Pub. Date Nov. 3, 2022.
Claims priority of application No. 21171246 (EP), filed on Apr. 29, 2021.
Prior Publication US 2024/0093257 A1, Mar. 21, 2024
This patent is subject to a terminal disclaimer.
Int. Cl. C12P 7/64 (2022.01); C07H 13/06 (2006.01); C08G 18/42 (2006.01); C11C 1/02 (2006.01); C12P 7/6481 (2022.01); C12P 19/14 (2006.01); C12P 19/44 (2006.01); C11D 1/66 (2006.01)

CPC C12P 19/44 (2013.01) [C12P 7/6481 (2013.01); C12P 19/14 (2013.01); C11D 1/662 (2013.01)]

17 Claims

1. A process to convert di-rhamnolipid to mono-rhamnolipid comprising, using an α-L-rhamnosidase enzyme which has a sequence identity of at least 70% with either SEQ ID. 1 or SEQ ID. 2;

wherein the process to convert di-rhamnolipid to mono-rhamnolipid comprises:

(a) contacting a starting di-rhamnolipid material with an α-L-rhamnosidase enzyme which is immobilised on a support by covalent bonding; and

(b) separating a produced mono-rhamnolipid from a reaction medium, side products, and a combination thereof.