US 12,371,706 B2
Methods of identifying, selecting, and producing anthracnose stalk rot resistant crops
Alyssa Marie Deleon, Des Moines, IA (US); Kevin A Fengler, Clive, IA (US); Shawn Thatcher, Urbandale, IA (US); Petra J Wolters, Kennett Square, PA (US); and Mark Timothy Jung, Urbandale, IA (US)
Assigned to PIONEER HI-BRED INTERNATIONAL, INC., Johnston, IA (US)
Appl. No. 17/636,060
Filed by PIONEER HI-BRED INTERNATIONAL, INC., Johnston, IA (US)
PCT Filed Aug. 17, 2020, PCT No. PCT/US2020/046665
§ 371(c)(1), (2) Date Feb. 17, 2022,
PCT Pub. No. WO2021/041077, PCT Pub. Date Mar. 4, 2021.
Claims priority of provisional application 62/890,729, filed on Aug. 23, 2019.
Prior Publication US 2022/0282338 A1, Sep. 8, 2022
Int. Cl. C12N 15/82 (2006.01); C12Q 1/6895 (2018.01)
CPC C12N 15/8282 (2013.01) [C12Q 1/6895 (2013.01); C12Q 2600/13 (2013.01); C12Q 2600/156 (2013.01)] 4 Claims
 
1. A method of introducing a heterologous NLR02 polynucleotide into a maize plant cell, wherein the NLR02 polynucleotide is associated with increased resistance to anthracnose stalk rot, the method comprising:
introducing the heterologous NLR02 polynucleotide into the maize plant cell, wherein the heterologous NLR02 polynucleotide is inserted into the maize plant genome at a non-natural location and comprises a polynucleotide sequence encoding a polypeptide comprising the sequence set forth in SEQ ID NO: 2.