US 12,371,702 B2
Improving agronomic characteristics in maize by modification of endogenous mads box transcription factors
Shane E Abbitt, Ankeny, IA (US); Mary J Frank, Des Moines, IA (US); Shai Lawit, Urbandale, IA (US); Rosana Melo, St Charles, MO (US); Mary A Rupe, Altoona, IA (US); Bo Shen, Johnston, IA (US); and Jingrui Wu, Johnston, IA (US)
Assigned to PIONEER HI-BRED INTERNATIONAL, INC., Johnston, IA (US)
Appl. No. 17/047,946
Filed by PIONEER HI-BRED INTERNATIONAL, INC., Johnston, IA (US)
PCT Filed Apr. 16, 2019, PCT No. PCT/US2019/027602
§ 371(c)(1), (2) Date Oct. 15, 2020,
PCT Pub. No. WO2019/204256, PCT Pub. Date Oct. 24, 2019.
Claims priority of provisional application 62/814,373, filed on Mar. 6, 2019.
Claims priority of provisional application 62/741,529, filed on Oct. 4, 2018.
Claims priority of provisional application 62/659,579, filed on Apr. 18, 2018.
Prior Publication US 2021/0155949 A1, May 27, 2021
Int. Cl. C12N 15/82 (2006.01); A01H 5/10 (2018.01)
CPC C12N 15/8241 (2013.01) [A01H 5/10 (2013.01)] 10 Claims
 
1. A maize plant cell comprising an introduced targeted genetic modification within an endogenous gene that encodes a polypeptide comprising an amino acid sequence that is at least 98% identical to the amino acid sequence of SEQ ID NO: 4, wherein the targeted genetic modification is an insertion of a heterologous promoter or an enhancer element at the genomic locus of the endogenous gene and wherein the targeted modification results in an increased expression level of the polynucleotide encoding the polypeptide.