US 12,371,681 B2
Means and methods for cleavage of zearalenone
Michaela Thamhesl, Goellersdorf (AT); Sebastian Fruhauf, Tulln (AT); Wulf-Dieter Moll, Stockerau (AT); Andreas Hoebartner, Obermeisling (AT); Gerd Schatzmayr, Tulln (AT); Eva Maria Binder, Tulln (AT); Markus Aleschko, Vienna (AT); and Martin Pfeffer, Tulln (AT)
Assigned to DSM Austria GmbH, Getzersdorf (AT)
Appl. No. 17/264,096
Filed by DSM Austria GmbH, Getzersdorf (AT)
PCT Filed Jul. 30, 2019, PCT No. PCT/EP2019/070434
§ 371(c)(1), (2) Date Jan. 28, 2021,
PCT Pub. No. WO2020/025580, PCT Pub. Date Feb. 6, 2020.
Claims priority of application No. 18186532 (EP), filed on Jul. 31, 2018.
Prior Publication US 2021/0371835 A1, Dec. 2, 2021
Int. Cl. C12N 9/14 (2006.01); A23K 20/189 (2016.01); A23K 50/30 (2016.01); A23K 50/60 (2016.01); A23K 50/75 (2016.01); A23L 29/00 (2016.01); A23L 33/00 (2016.01); A23L 33/10 (2016.01); A61K 38/46 (2006.01)
CPC C12N 9/14 (2013.01) [A23K 20/189 (2016.05); A23K 50/30 (2016.05); A23K 50/60 (2016.05); A23K 50/75 (2016.05); A23L 29/06 (2016.08); A23L 33/10 (2016.08); A23L 33/40 (2016.08); A61K 38/46 (2013.01); A23V 2002/00 (2013.01)] 11 Claims
OG exemplary drawing
 
1. A method for increasing the stability of an α/β-hydrolase, which α/β-hydrolase comprises a sequence corresponding to positions 145 to 218 of SEQ ID NO: 1 or a sequence having 80% or more sequence identity to a sequence corresponding to positions 145 to 218 of SEQ ID NO: 1, comprising
substituting at least one amino acid
at a position corresponding to position 160 to 205 of SEQ ID NO: 1, or
at a position corresponding to position 159 to 204 of SEQ ID NO: 2, or
at a position corresponding to position 160 to 205 of SEQ ID NO: 3, 4, or 5, or
at a position corresponding to position 176 to 222 of SEQ ID NO: 6,
wherein the amino acid(s) are substituted with an amino acid which has a more negative hydropathy index than the substituted amino acid,
wherein the hydropathy index is determined by the Kyte and Doolittle hydropathy index, thereby obtaining an α/β-hydrolase with increased stability compared to the α/β-hydrolase before substituting said amino acid(s).