US 12,043,839 B2
Methods and compositions for targeting RNA polymerases and non-coding RNA biogenesis to specific loci
Steve E. Jacobsen, Agoura Hills, CA (US); Javier Gallego-Bartolomé, Beverly Hills, CA (US); and Ashot Papikian, Glendale, CA (US)
Assigned to The Regents of the University of California, Oakland, CA (US)
Filed by The Regents of the University of California, Oakland, CA (US)
Filed on Feb. 14, 2022, as Appl. No. 17/671,466.
Application 17/671,466 is a continuation of application No. 16/304,113, granted, now 11,286,493, previously published as PCT/US2017/034844, filed on May 26, 2017.
Claims priority of provisional application 62/342,814, filed on May 27, 2016.
Claims priority of provisional application 62/450,504, filed on Jan. 25, 2017.
Prior Publication US 2022/0275386 A1, Sep. 1, 2022
Int. Cl. C12N 15/82 (2006.01); C07K 16/00 (2006.01); C12N 9/10 (2006.01); C12N 9/12 (2006.01)
CPC C12N 15/8216 (2013.01) [C07K 16/00 (2013.01); C12N 9/1007 (2013.01); C12N 9/1241 (2013.01); C12N 15/8218 (2013.01); C07K 2317/622 (2013.01); C07K 2319/81 (2013.01); C12N 2310/14 (2013.01); C12N 2310/20 (2017.05); C12N 2310/3521 (2013.01)] 16 Claims
 
1. A method for reducing expression of a target nucleic acid in a plant, comprising:
a) providing a plant comprising a recombinant epigenetic regulator polypeptide, wherein said recombinant polypeptide comprises (1) an H3K4 demethylase polypeptide selected from the group consisting of a JMJ14 polypeptide and a JMJ18 polypeptide, wherein the JMJ14 polypeptide comprises an amino acid sequence having at least 95% amino acid identity to SEQ ID NO: 80 and the JMJ18 polypeptide comprises an amino acid sequence having at least 95% amino acid identity to SEQ ID NO: 376, and (2) a heterologous DNA-binding domain capable of binding a target nucleic acid, and;
b) growing the plant under conditions whereby the recombinant epigenetic regulator polypeptide is targeted to the target nucleic acid, thereby reducing expression of the target nucleic acid.